DamID

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DamID^[1] (DNA adenine methyltransferase identification) is a molecular biology protocol used to map the binding sites of DNA-binding proteins in eukaryotes. DamID identifies binding sites by expressing the proposed DNA-binding protein as a fusion protein with DNA methyltransferase. Binding of the protein of interest to DNA localizes the methyltransferase in the region of the binding site. Adenosine methylation does not occur naturally in eukaryotes and it can therefore any region of DNA with adenine methylation has been caused by the fusion protein, implying it is located near a binding site. DamID is an alternate method to ChIP-on-chip.

[edit] Notes

1. ^ http://www.ncbi.nlm.nih.gov/pubmed/10748524 Van Steensel and Henikof, 2000

[edit] External links

• Detection of in vivo protein-DNA interactions using DamID in mammalian cells. Nature protocols,2007
• DamID: Mapping of In Vivo Protein–Genome Interactions Using Tethered DNA Adenine Methyltransferase.
• Frequently asked questions about DamID
• DamID, a new tool for studying plant chromatin profiling in vivo, and its use to identify putative LHP1 target loci