Cytochalasin B

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Cytochalasin B
Other names	Phomin
Molecular formula	C29H37NO5
Identifiers
CAS number	[14930-96-2]
Properties
Molar mass	479.6
Solubility in water	1.280 mg/L
Hazards
MSDS	Cytochalasin B MSDS from Fermentek
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) Infobox disclaimer and references

Cytochalasin B is a cell-permeable mycotoxin. It inhibits cytoplasmic division by blocking the formation of contractile microfilaments. It inhibits cell movement and induces nuclear extrusion. Cytochalasin B shortens actin filaments by blocking monomer addition at the fast-growing end of polymers. Cytochalasin B inhibits glucose transport and platelet aggregation. It blocks adenosine-induced apoptotic body formation without affecting activation of endogenous ADP-ribosylation in leukemia HL-60 cells. It is also used in cloning through nuclear transfer. Here enucleated recipient cells are treated with Hoechst stain containing cytochalsin B. Cytochalasin B makes the walls of the oocytes smoother and makes it easy for micro-manipulation so that the zona pellucida is not so rigid and can be easily pierced by the micro-needles.

This alkaloid is isolated from a fungus, Helminthosporium dermatioideum.

[edit] References

• Merck Index 13, 2819.
• Tanaka, Y., et al. 1994. Exp. Cell Res. 213, 242.
• Theodoropoulos, P.A., et al. 1994. Biochem. Pharmacol. 47, 1875.
• Benya, P.D., and Padilla, S.R. 1993. Exp. Cell Res. 204, 268.

[edit] External links