Cyanohydrin beta-glucosyltransferase

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In enzymology, a cyanohydrin beta-glucosyltransferase (EC 2.4.1.85) is an enzyme that catalyzes the chemical reaction

UDP-D-glucose + (S)-4-hydroxymandelonitrile $\rightleftharpoons$ UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside

Thus, the two substrates of this enzyme are UDP-D-glucose and (S)-4-hydroxymandelonitrile, whereas its two products are UDP and (S)-4-hydroxymandelonitrile beta-D-glucoside.

This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is UDP-D-glucose:(S)-4-hydroxymandelonitrile beta-D-glucosyltransferase. Other names in common use include uridine diphosphoglucose-p-hydroxymandelonitrile, glucosyltransferase, UDP-glucose-p-hydroxymandelonitrile glucosyltransferase, uridine diphosphoglucose-cyanohydrin glucosyltransferase, uridine diphosphoglucose:aldehyde cyanohydrin, beta-glucosyltransferase, UDP-glucose:(S)-4-hydroxymandelonitrile beta-D-glucosyltransferase, UGT85B1, and UDP-glucose:p-hydroxymandelonitrile-O-glucosyltransferase. This enzyme participates in tyrosine metabolism and cyanoamino acid metabolism.

[edit] References

IUBMB entry for 2.4.1.85
BRENDA references for 2.4.1.85 (Recommended.)
PubMed references for 2.4.1.85
PubMed Central references for 2.4.1.85
Google Scholar references for 2.4.1.85
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Busk PK, Moller BL (2002). "Dhurrin synthesis in sorghum is regulated at the transcriptional level and induced by nitrogen fertilization in older plants". Plant. Physiol. 129: 1222–31. doi:10.1104/pp.000687. PMID 12114576.
BL, Bak S (2005). "Metabolic engineering of dhurrin in transgenic Arabidopsis plants with marginal inadvertent effects on the metabolome and transcriptome". Proc. Natl. Acad. Sci. U. S. A. 102: 1779–84. doi:10.1073/pnas.0409233102. PMID 15665094.