CPSF4

From Wikipedia, the free encyclopedia

Cleavage and polyadenylation specific factor 4, 30kDa

PDB rendering based on 2d9n.

Available structures: 2d9n

Identifiers

Symbol(s)

CPSF4; CPSF30; NAR; NEB1

External IDs

OMIM: 603052 MGI: 1861602 HomoloGene: 38216

Gene ontology
Molecular Function:	• RNA binding • zinc ion binding • metal ion binding
Cellular Component:	• nucleus
Biological Process:	• mRNA processing

RNA expression pattern

More reference expression data

Orthologs

Human

Mouse

Refseq

NM_001081559 (mRNA)
NP_001075028 (protein)

XM_001004463 (mRNA)
XP_001004463 (protein)

Location

Chr 7: 98.87 - 98.89 Mb

Chr 5: 145.42 - 145.44 Mb

Pubmed search

[1]

[2]

Cleavage and polyadenylation specific factor 4, 30kDa, also known as CPSF4, is a human gene.^[1]

Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene.^[1]

[edit] References

^ ^a ^b Entrez Gene: CPSF4 cleavage and polyadenylation specific factor 4, 30kDa.

[edit] Further reading

Jenny A, Hauri HP, Keller W (1994). "Characterization of cleavage and polyadenylation specificity factor and cloning of its 100-kilodalton subunit.". Mol. Cell. Biol. 14 (12): 8183–90. PMID 7969155.
Thuresson AC, Aström J, Aström A, et al. (1994). "Multiple forms of poly(A) polymerases in human cells.". Proc. Natl. Acad. Sci. U.S.A. 91 (3): 979–83. PMID 8302877.
Hillier LD, Lennon G, Becker M, et al. (1997). "Generation and analysis of 280,000 human expressed sequence tags.". Genome Res. 6 (9): 807–28. PMID 8889549.
McCracken S, Fong N, Yankulov K, et al. (1997). "The C-terminal domain of RNA polymerase II couples mRNA processing to transcription.". Nature 385 (6614): 357–61. doi:10.1038/385357a0. PMID 9002523.
Barabino SM, Hübner W, Jenny A, et al. (1997). "The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins.". Genes Dev. 11 (13): 1703–16. PMID 9224719.
Nemeroff ME, Barabino SM, Li Y, et al. (1998). "Influenza virus NS1 protein interacts with the cellular 30 kDa subunit of CPSF and inhibits 3'end formation of cellular pre-mRNAs.". Mol. Cell 1 (7): 991–1000. PMID 9651582.
de Vries H, Rüegsegger U, Hübner W, et al. (2000). "Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors.". EMBO J. 19 (21): 5895–904. doi:10.1093/emboj/19.21.5895. PMID 11060040.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMID 12477932.
Scherer SW, Cheung J, MacDonald JR, et al. (2003). "Human chromosome 7: DNA sequence and biology.". Science 300 (5620): 767–72. doi:10.1126/science.1083423. PMID 12690205.
Kaufmann I, Martin G, Friedlein A, et al. (2005). "Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase.". EMBO J. 23 (3): 616–26. doi:10.1038/sj.emboj.7600070. PMID 14749727.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMID 15489334.
Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network.". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
Oh JH, Yang JO, Hahn Y, et al. (2006). "Transcriptome analysis of human gastric cancer.". Mamm. Genome 16 (12): 942–54. doi:10.1007/s00335-005-0075-2. PMID 16341674.
Twu KY, Kuo RL, Marklund J, Krug RM (2007). "The H5N1 influenza virus NS genes selected after 1998 enhance virus replication in mammalian cells.". J. Virol. 81 (15): 8112–21. doi:10.1128/JVI.00006-07. PMID 17522219.