Talk:Cordyceps

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Fig. 4. Effect of cordycepin on LPS-induced COX-2 protein expression, and TNF-α mRNA expression. (A) RAW 264.7 cells (4×106 cells/well) were pretreated with the indicated concentrations of cordycepin for 30 min before incubation with LPS (1 μg/ml) for 16 h. The cells were lysed, and the lysates were analyzed by immunoblotting used anti-COX-2. The blot was stripped of the bound antibody and reprobed with anti-β actin to confirm equal loading. (B) Raw cells were pretreated with cordycepin for 30 min and then treated with LPS in the presence of cordycepin for 8 h. Total RNAwas isolated and used for TNF- α or actin RT-PCR. Total RNA was prepared and RT-PCR analysis was performed as described in Materials and methods.