Chloro-5-substituted adamantyl-1,2-dioxetane phosphate

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CSPD (C₁₈H₂₀ClO₇PNa₂) or disodium 3-(4-methoxyspiro{1,2-dioxetane-3,2'-(5'-chloro)tricyclo[3.3.1.1^3,7]decan}-4-yl)phenyl phosphate or Chloro-5-substituted adamantyl-1,2-dioxetane phosphate is a reagent that is used in enhanced chemiluminescence ELISA kits.

The enzyme alkaline phosphatase dephosphorylates CSPD. The resulting intermediate phenolate ion emits chemiluminescent turquoise-coloured light (λ_max = 477 nm), thereby splitting itself in two components (adamantane and dioxetane).

(A = adamantyl, M = methoxy, P = phenyl , P = phosphate, D = dioxetane

The Bayer energy that is stored in the ring, is emitted as 477 nm light.

[edit] External links

• http://web.mit.edu/7.02/resources/CSPD.pdf
• http://www.jenobiotech.com/techsupport/Protocol/ELISA1-15.pdf