Chinese hamster ovary cell

From Wikipedia, the free encyclopedia

Chinese Hamster Ovary cells (CHO cells) are a cell line derived from Chinese Hamster ovary cells. They are often used in biological and medical research. They were introduced in the 1960s and are used in a cultured monolayer in culture flasks. They require the amino acid proline in their culture medium; this property makes them ideal for genetics research.^{[citation needed]} CHO cells are used in studies of genetics, toxicity screening, nutrition and gene expression particularly expression of recombinant proteins. Today, CHO cells are the most commonly used mammalian hosts for industrial production of recombinant protein therapeutics.

[edit] See also

• Cell culture
• Drug development
• Pre-clinical development
• Caco-2

[edit] References

Tjio J. H., Puck T. T., Genetics of somatic mammalian cells. II. chromosomal constitution of cells in tissue culture. J. Exp. Med. 1958; 108: 259-271.

Jayapal K. P., Wlaschin K. F., Yap M. G. S., Hu W-S., Recombinant protein therapeutics from CHO cells - 20 years and counting. Chem. Eng. Prog. 2007; 103(7) 40-47.

[edit] External links

• http://hugroup.cems.umn.edu/HuGroup/PDFs/Jayapal_et_al_CHO_Review.pdf
• Phase contrast images of CHO cells
• Article from 1958 on culturing euploid cells
• http://www.biotech.ist.unige.it/cldb/cl721.html