Chemical tests in mushroom identification

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Of the Chemical tests in mushroom identification the most useful are Melzer's and Potassium hydroxide. A list of tests follows.

1 Ammonia
2 Iron Salts
3 Meixner Test for Amatoxins
4 Melzer's Reagent
5 Paradiaminobenzaldehyde
6 Phenol
7 Potassium Hydroxide
8 Schaeffer's Test
9 Sulpho-vanillin
10 References
11 External links

[edit] Ammonia

Household ammonia can be used.

[edit] Iron Salts

Used in Russula identification.

[edit] Meixner Test for Amatoxins

Uses concentrated hydrochloric acid and newspaper to test for the deadly poisonous amatoxins found in some species of Amanita, Lepiota, and Galerina.

[edit] Melzer's Reagent

Melzer's Reagent can be used to test spores for amyloid, nonamyloid, dextrinoid.

Spores that stain bluish-gray to bluish-black are amyloid
Spores that stain brown to reddish-brown are dextrinoid

[edit] Paradiaminobenzaldehyde

In the genus Lyophyllum the lamellae usually bluing with Paradiaminobenzaldehyde (PDAB).

[edit] Phenol

A 2-3% aqueous solution of phenol gives a color change in some species when applied to cap or stem.

[edit] Potassium Hydroxide

A 5-10% solution of Potassium hydroxide (KOH) gives a color change in some species:

In Agaricus, some species turn yellow with KOH
Distinctive change for some species of Cortinarius

[edit] Schaeffer's Test

Uses aniline and nitric acid on Agaricus.

[edit] Sulpho-vanillin

Made from Sulphuric acid (H₂SO₄) and vanillin (vanilla). Used in Russula indentification.

[edit] References

Arora, David "Mushrooms Demystified" 2nd Edition, Ten Speed Press, Berkeley, 1986
Jordan, Michael "The Encyclopedia of Fungi of Britain and Europe" Frances Lincoln 2004
Kuo, Michael "100 Edible Mushrooms", University of Michigan Press, Ann Arbor 2007
Largent, David L., Baroni, Timothy J. "How to Identify Mushrooms to Genus VI: Modern Genera" Mad River Press 1988