Cellulase
From Wikipedia, the free encyclopedia
Cellulase refers to a class of enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze the cellulolysis (or hydrolysis) of cellulose. However, there are also cellulases produced by other types of organisms such as plants and animals. Several different kinds of cellulases are known, which differ structurally and mechanistically. The EC number for this group of enzymes is EC 3.2.1.4.
Reaction: Endohydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Other names: Endoglucanase. Endo-1,4-beta-glucanase. Carboxymethyl cellulase. Endo-1,4-beta-D-glucanase. Beta-1,4-glucanase. Beta-1,4-endoglucan hydrolase. Celludextrinase. Avicelase.
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[edit] Types and action
Five general types of cellulases based on the type of reaction catalyzed:
- Endo-cellulase breaks internal bonds to disrupt the crystalline structure of cellulose and expose individual cellulose polysaccharide chains
- Exo-cellulase cleaves 2-4 units from the ends of the exposed chains produced by endocellulase, resulting in the tetrasaccharides or disaccharide such as cellobiose. There are two main types of exo-cellulases (or cellobiohydrolases, abbreviate CBH) - one type working processively from the reducing end, and one type working processively from the non-reducing end of cellulose.
- Cellobiase or beta-glucosidase hydrolyses the exo-cellulase product into individual monosaccharides.
- Oxidative cellulases that depolymerize cellulose by radical reactions, as for instance cellobiose dehydrogenase (acceptor).
- Cellulose phosphorylases that depolymerize cellulose using phosphates instead of water.
- Cellulase also does not dissolve certain chemicals found in certain fruits, such as bananas, grapefruits, or apples.
In the most familiar case of cellulase activity, the enzyme complex breaks down cellulose to beta-glucose. This type of cellulase is produced mainly by symbiotic bacteria in the ruminating chambers of herbivores. Aside from ruminants, most animals (including humans) do not produce cellulase in their bodies, and are therefore unable to use most of the energy contained in plant material. Enzymes which hydrolyze Hemicellulose are usually referred to as hemicellulase and are usually classified under cellulase in general. Enzymes that cleave lignin are occasionally classified as cellulase, but this is usually considered erroneous.
Within the above types there are also progressive (also known as processive) and non-progressive types. Progressive cellulase will continue to interact with a single polysaccharide strand, non-progressive cellulase will interact once then disengage and engage another polysaccharide strand.
Most fungal cellulases have a two-domain structure with one catalytic domain, and one cellulose binding domain, that are connected by a flexible linker. This structure is adaption for working on an insoluble substrate and it allows the enzyme to diffuse two-dimensionally on a surface in a caterpillar way. However, there are also cellulases (mostly endoglucanases) that lacks cellulose binding domain. These enzymes might have a swelling function.
[edit] Mechanism of cellulolysis
[edit] Uses
Cellulase is used for commercial food processing in coffee. It performs hydrolysis of cellulose during drying of beans. Furthermore, cellulases are widely used in textile industry and in laundry detergents. They have also been used in the pulp and paper industry for various purposes, and they are even used for pharmaceutical applications. Cellulase is used in the fermentation of biomass into biofuels, although this process is relatively experimental at present. Cellulase is used as a treatment for Phytobezoars, a form of cellulose bezoar found in the human stomach.
[edit] References
- Chapin III, F.S., P.A. Matson, H.A. Mooney. Principles of Terrestrial Ecosystem Ecology. Springer-Verlag New York, NY. 2002
- The Merck Manual of Diagnosis and Therapy, Chapter 24
[edit] See also
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