Talk:CDNA library

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Article Grading: The following comments were left by the quality and importance raters: (edit · history · refresh · how to use this template)

I was concerned that the article said a cDNA library contains all the mRNAs in a cell. A major problem with cDNA libraries is that they often do not contain all the mRNAs, but rather a random sampling. I did not have the time to do a real revision of this article -- I just changed the first sentence. Probably a bad idea, but I would urge a careful review of it. I will try to post more detailed comments soon.

Michael.brent 02:22, 7 September 2007 (UTC)

Contents

[edit] Suggestion

My grammar is not good and I don't feel comfortable editing this article but someone should explain how the cDNA libraries contain only introns and are therefore much easier to work with than the complete genome.

Thanks.


[edit] response to suggestion

cDNA librarys are made from mRNA which contains only exons. The introns have been spliced out and will not be found in a cDNA library.


[edit] bullcrap

RNases which can be found even on the skin? The outer layer of the dermis, the epidermis is dead material, and consists mostly out of ceratine. Shouldn`t this be in the epithelial cells, but this would come as no wonder, but besides what has RNase and skin todo anyways in this article.193.170.48.34 16:07, 26 June 2006 (UTC)

[edit] response to bullcrap

HAHAHAHA learn a bit more about the topic before writing such things here! HAHAHAHA RNases ARE everywhere, you can sneeze them out, and your entire experiment would be destroyed, if you work with RNA. Yes, they can be on your hands, as so many other things can be on your hands, a "zoo" of microorganisms for example.

[edit] dudees, this article is too tiny!!!

write more on this topic pls. my english is not good enough, and i have exam time anyway. PLEASE!

[edit] Article Plan

I've only recently started work on the article. After reviewing it, I realised it needed a lot of work. A lot more work. So, here's the thing. Perhaps we can discuss how this article should be arranged. Here's my suggestion of what I think should be included...

  • Intro
  • cDNA Library construction
    • mRNA extraction
    • Creating cDNA
    • Splicing plasmids
    • Bacterial transformation
  • cDNA uses (must include high-profile examples)
    • Advantages and disadvantages

What do you guys think?

Wolf ODonnell 11:39, 8 November 2007 (UTC)

I started some re-working of the article. I cleaned it up a little bit and removed the "expert-needed template". I'll keep working and try to get some references and additional info in the near future. Schu1321 (talk) 08:31, 12 May 2008 (UTC)