CDK2AP1

From Wikipedia, the free encyclopedia

CDK2-associated protein 1

Identifiers

CDK2AP1; DOC1; DORC1; ST19; doc-1; p12DOC-1

External IDs

OMIM: 602198 MGI: 1202069 HomoloGene: 3411

Gene ontology
Molecular Function:	• DNA binding • signal transducer activity
Cellular Component:	• nucleus • cytoplasm
Biological Process:	• S phase of mitotic cell cycle • DNA-dependent DNA replication • protein amino acid phosphorylation • cell cycle • negative regulation of progression through cell cycle

RNA expression pattern

More reference expression data

Orthologs

Human

Mouse

Refseq

NM_004642 (mRNA)
NP_004633 (protein)

XM_991893 (mRNA)
XP_996987 (protein)

Location

Chr 12: 122.31 - 122.32 Mb

Chr 11: 48.67 - 48.67 Mb

Pubmed search

[1]

[2]

CDK2-associated protein 1, also known as CDK2AP1, is a human gene.^[1]

The protein encoded by this gene is a specific CDK2-associated protein, which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggested the regulatory role in DNA replication during S phase of the cell cycle. A similar gene in hamster was isolated from, and functions as a growth suppressor of normal keratinocytes.^[1]

[edit] References

^ ^a ^b Entrez Gene: CDK2AP1 CDK2-associated protein 1.

[edit] Further reading

Daigo Y, Suzuki K, Maruyama O, et al. (1997). "Isolation, mapping and mutation analysis of a human cDNA homologous to the doc-1 gene of the Chinese hamster, a candidate tumor suppressor for oral cancer.". Genes Chromosomes Cancer 20 (2): 204–7. PMID 9331572.
Tsuji T, Duh FM, Latif F, et al. (1998). "Cloning, mapping, expression, function, and mutation analyses of the human ortholog of the hamster putative tumor suppressor gene Doc-1.". J. Biol. Chem. 273 (12): 6704–9. PMID 9506968.
Matsuo K, Shintani S, Tsuji T, et al. (2000). "p12(DOC-1), a growth suppressor, associates with DNA polymerase alpha/primase.". FASEB J. 14 (10): 1318–24. PMID 10877824.
Shintani S, Ohyama H, Zhang X, et al. (2000). "p12(DOC-1) is a novel cyclin-dependent kinase 2-associated protein.". Mol. Cell. Biol. 20 (17): 6300–7. PMID 10938106.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMID 12477932.
Yuan Z, Sotsky Kent T, Weber TK (2003). "Differential expression of DOC-1 in microsatellite-unstable human colorectal cancer.". Oncogene 22 (40): 6304–10. doi:10.1038/sj.onc.1206609. PMID 13679870.
Hu MG, Hu GF, Kim Y, et al. (2004). "Role of p12(CDK2-AP1) in transforming growth factor-beta1-mediated growth suppression.". Cancer Res. 64 (2): 490–9. PMID 14744761.
Buajeeb W, Zhang X, Ohyama H, et al. (2004). "Interaction of the CDK2-associated protein-1, p12(DOC-1/CDK2AP1), with its homolog, p14(DOC-1R).". Biochem. Biophys. Res. Commun. 315 (4): 998–1003. doi:10.1016/j.bbrc.2004.02.003. PMID 14985111.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMID 15489334.
Kim Y, Ohyama H, Patel V, et al. (2005). "Mutation of Cys105 inhibits dimerization of p12CDK2-AP1 and its growth suppressor effect.". J. Biol. Chem. 280 (24): 23273–9. doi:10.1074/jbc.M412929200. PMID 15840587.
Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network.". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
Peng H, Shintani S, Kim Y, Wong DT (2007). "Loss of p12CDK2-AP1 expression in human oral squamous cell carcinoma with disrupted transforming growth factor-beta-Smad signaling pathway.". Neoplasia 8 (12): 1028–36. doi:10.1593/neo.06580. PMID 17217620.
Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry.". Mol. Syst. Biol. 3: 89. doi:10.1038/msb4100134. PMID 17353931.
Shin J, Yuan Z, Fordyce K, et al. (2007). "A del T poly T (8) mutation in the 3' untranslated region (UTR) of the CDK2-AP1 gene is functionally significant causing decreased mRNA stability resulting in decreased CDK2-AP1 expression in human microsatellite unstable (MSI) colorectal cancer (CRC).". Surgery 142 (2): 222–7. doi:10.1016/j.surg.2007.04.002. PMID 17689689.