Brefeldin A
From Wikipedia, the free encyclopedia
| Brefeldin A | |
|---|---|
 |
|
| IUPAC name | γ,4-Dihydroxy-2-(6-hydroxy-1-heptenyl)-4-cyclopentanecrotonic acid λ-lactone |
| Identifiers | |
| CAS number | [20350-15-6] |
| PubChem | |
| Properties | |
| Molecular formula | C16H24O4 |
| Molar mass | 280.36 |
| Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) Infobox disclaimer and references |
|
Brefeldin A is a lactone antibiotic produced by fungal organisms such as Eupenicillium brefeldianum. Brefeldin A interferes with anterograde protein transport from the endoplasmic reticulum to the Golgi apparatus by inhibiting transport in Golgi, which leads to proteins accumulating inside the ER. In mammalian and yeast cells, the main target of brefeldin A appears to be a certain type of GTP-exchange factors responsible for activating a GTPase called Arf1p ; in turn, Arf1p is involved in the formation of transport vesicles by recruiting coat proteins to intracellular membranes. Brefeldin A was initially isolated as an anti-viral antibiotic [1] but is now primarily used in biological research to study protein transport.
[edit] Physical data
- Molecular formula: C16H24O4
- Appearance: White to off-white crystalline powder
- Solubility: Clear colorless solution at 10mg/ml Dichloromethane.
- Clear colorless solution at 10mg/ml Methanol.
- Melting point: 297°C-205°C
Brefeldin A affects COPI coat formation, which is involved in retrograde transport. It inhibits ArfI activation, prohibiting recruitment of COPI coat proteins to the budding vessicle by binding to its guanine exchange factor.
