Bis-tris propane

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Bis-tris propane


IUPAC name	2,2'-(Propane-1,3- diyldiimino)bis[2-(hydroxymethyl) propane-1,3-diol]
Other names	Bis-tris propane
Identifiers
CAS number	[64431-96-5]
SMILES	OCC(CO)(CO)NCCCNC(CO)(CO)CO
Properties
Molecular formula	C₁₁H₂₆N₂O₆
Molar mass	282.34 g/mol
Density	? g/cm³
Melting point	164-165 °C
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) Infobox disclaimer and references

Bis-tris propane, or 1,3-bis(tris(hydroxymethyl)methylamino)propane, is a chemical substance that is used in buffer solutions. It is a white to off-white crystalline powder that is soluble in water. It has a wide buffering range, from 6 to 9.5 due to its two pK_a values which are close in value. This buffer is primarily used in biochemistry and molecular biology.

1 Applications
2 See also
3 References
4 External Links

[edit] Applications

A review of DNA polymerase fidelity cites bis-tris propane as a suitable buffer for polymerase chain reaction (PCR).^[1] Bis-Tris propane has also been used with HCl buffer for stabilization of farnesyl diphosphate isolated from a strain of Saccharomyces cerevisiae.^[2] It has also been used in a study of the effects of buffer identity on electric signals of light-excited bacteriorhodospin.^[3] Use of Bis-Tris propane has also been documented in an investigation of the MgATPase activity of the myosin subfragment 1 monomer.^[4] The effect of buffer identity on the kinetics of the restriction enzyme EcoRV has been studied in various buffers, including Bis-Tris propane.^[5]

[edit] See also

[edit] References

^ Eckert K.A., and Kunkel, T.A.: "DNA polymerase fidelity and the polymerase chain reaction" "PCR Methods Appl", 1(1):17-24.
^ Song, L. "Detection of farnesyl diphosphate accumulation in yeast ERG9 mutants" "Anal. Biochem.", 317(2):180-185.
^ Toth-Boconadi, R., et al., "Buffer effects on electric signals of light-excited bacteriorhodospin" "Biophys. J.", 78(6):3170-3177.
^ Bachouchi, N., "MgATPase activity of myosin subfragment 1. The dimer is more active than the monomer" "J. Mol. Biol." 191(2):247-254.
^ Wenner, J.R., and Bloomfield, V.A., "Buffer effects on EcoRV kinetics as measured by fluorescent staining and digital imaging of plasmid cleavage." "Anal. Biochem." 268(2):201-212.