Bicinchoninic acid
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Bicinchoninic acid | |
---|---|
IUPAC name | 2-(4-carboxyquinolin-2-yl)quinoline-4-carboxylic acid |
Other names | Bicinchoninic acid 4,4'-Dicarboxy-2,2'-Biquinoline |
Identifiers | |
CAS number | [1245-13-2] |
Properties | |
Molecular formula | (HO2CC9H5N)2 |
Molar mass | 344.33 g/mol |
Appearance | Cream powder. Characteristic odor. |
Density | Solid in pure form. Density not available. |
Melting point |
365 to 367 °C |
Boiling point |
Not available. |
Solubility in water | Partially soluble in cold water, hot water. |
Hazards | |
MSDS | External MSDS |
Main hazards | Will irritate eyes and mucous membranes. The chemical, physical and toxicological properties have not been fully investigated. Presume moderate toxicity internally. LD/TD no data. OSHA PEL/ACGIH TLV not established. No evidence of carcinogenicity. |
NFPA 704 | |
Flash point | N/A |
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) Infobox disclaimer and references |
Bicinchoninic acid is a weak acid composed of two carboxylated quinoline rings.
Bicinchoninic acid is most commonly employed by biochemists in the bicinchoninic acid assay, which is used to determine the total level of protein in a solution. In this assay, two molecules of bicinchoninic acid chelate a single Cu1+ ion, forming a purple water-soluble complex that strongly aborbs light at 562 nm.
[edit] References
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