Betulinic acid

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Betulinic acid
IUPAC name (3β)-3-Hydroxy-lup-20(29)-en-28-oic acid
Other names Betulic acid
Mairin
Identifiers
CAS number [472-15-1]
PubChem 2371
SMILES CC(=C)C1CCC2(C1C3CCC4C5(CCC(C(C5CCC4(C3(CC2)C)C)(C)C)O)C)C(=O)O
Properties
Molecular formula C30H48O3
Molar mass 456.7 g/mol
Melting point

316-318 °C
Except where noted otherwise, data are given for
materials in their standard state
(at 25 °C, 100 kPa)
Infobox disclaimer and references

Betulinic acid is a naturally occurring pentacyclic triterpenoid which has anti-retroviral, anti-malarial, and anti-inflammatory properties, as well as a more recently discovered potential as an anticancer agent, by inhibition of topoisomerase. [1] It is found in the bark of several species of plants, including the Ber tree (Ziziphus mauritiana), the white birch (Betula pubescens), [2] and many other plants such as the tropical carnivorous plants Triphyophyllum peltatum and Ancistrocladus heyneanus, Diospyros leucomelas a member of the persimmon family, Tetracera boiviniana, the jambul (Syzygium formosanum), [3] flowering quince (Chaenomeles sinensis), [4] and Pulsatilla chinensis. [5]

Contents

[edit] Anti-tumor activity

In 1995, betulinic acid was reported as a selective inhibitor of human melanoma. [6] Then it was demonstrated, that betulinic acid induces apoptosis in human melanoma in vitro and in vivo model systems.[7] Currently it is undergoing development with assistance from the Rapid Access to Intervention Development program of the National Cancer Institute. [2] Also betulinic acid was found active against neuroectodermal (neuroblastoma, medulloblastoma, Ewing's sarcoma[8]) and malignant brain tumors,[3][9] ovarian carcinoma,[3] in human leukemia HL-60 cells,[5] malignant head and neck squamous cell carcinoma SCC25 and SCC9 cell lines. [10] In contrast, epithelial tumors, such as breast carcinoma, colon carcinoma, small cell lung carcinoma and renal cell carcinoma as well as T-cell leukemia cells were completely refractory to treatment with betulinic acid.[8]

[edit] Mode of action

Regarding the mode of action of betulinic acid, little is known about its antiproliferative and apoptosis-inducing mechanisms. In neuroectodermal tumor cells betulinic acid–induced apoptosis is accompanied by caspase activation, mitochondrial membrane alterations and DNA fragmentation.[8][10] Caspases are produced as inactive proenzymes, which are proteolytically processed to their active forms. These proteases can cooperate in proteolytic cascades, in which caspases activate themselves and each other. The initiation of the caspases cascade may lead to the activation of endonucleases like caspase-activated DNAase (CAD). After activation CAD contributes to DNA degradation.[10] Betulinic acid induces apoptosis by direct effects on mitochondria, leading to cytochrome-c release, which in turn regulates the "downstream" caspase activation.[10] Betulinic acid bypasses resistance to CD95 and doxorubicin-mediated apoptosis, due to different molecular mechanism of betulinic acid-induced apoptosis.

Controversial is a role of p53 in betulinic acid-induced apoptosis. Fulda suggested p53-independent mechanism of the apoptosis, basing on fact of no accumulation of wild-type p53 detected upon treatment with the betulinic acid, whereas wild-type p53 protein strongly increased after treatment with doxorubicin.[8] The suggestion is supported by study of Raisova.[11] On the other hand Rieber suggested that betulinic acid exerts its inhibitory effect on human metastatic melanoma partly by increasing p53.[12]

The study also demonstrated preferential apoptotic effect of betulinic acid on C8161 metastatic melanoma cells, with greater DNA fragmentation and growth arrest and earlier loss of viability than their non-metastatic C8161/neo 6.3 counterpart.[12] Comparing the betulinic acid with other treatment modes, Zuco demonstrated that it was more than 10 times less potent than doxorubicin (IC50 4.5 μg/ml Vs IC50 0.21-034 μg/ml in doxorubicin) and showed an in vitro antiproliferative activity against melanoma and non-melanoma cell lines, including those resistant to doxorubicin. On the human normal dermatoblast cell line betulinic acid was 2-5 times less toxic than doxorubicin.[3] The ability of betulinic acid to induce two different effects (cytotoxic and cytostatic) on two clones derived from the same human melanoma metastasis suggests that the development of clones resistant to this agent will be more unlikely, than that to conventional cytotoxic drugs. Moreover in spite of the lower potency compared with doxorubicin betulinic acid seems to be selective for tumor cells with minimal toxicity against normal cells.[3] The effect of betulinic acid on melanoma cell lines is stronger than its growth-inhibitory effect on primary melanocytes.[13]Study of combination of betulinic acid with γ-irradiation showed clearly additive effects, and indicates that they differ in their mode of action.[13]

[edit] Anticancer derivatives

A major inconvenience for the future clinical development of betulinic acid and analogues resides in their poor solubility in aqueous media like blood serum and polar solvents used for bioassays. To circumvent this problem of hydrosolubility and to enhance pharmacological properties, many derivatives were synthesized and evaluated for cytotoxic activity. A study showed that C-20 modifications involve the loss of cytotoxicity. Another study demonstrated the importance of the presence of the COOH group since compounds substituted at this position like lupeol and methyl betulinate were less active on human melanoma than betulinic acid. Moreover, some C-28 amino acids and C-3 phthalates derivatives exhibited higher cytotoxic activity against cancer cell lines with improved selective toxicity and water solubility. Chatterjee and co-workers obtained the 28-O-β-D-glucopyranoside of betulinic acid by microbial transformation with Cunninghamella species while Baglin and co-workers obtained it by organic synthesis. This glucoside did not exhibit any significant in vitro activity on human melanoma (MEL-2) and human colorectal adenocarcinoma (HT-29) cell lines which confirms the importance of the carboxylic acid function to preserve the cytotoxicity. Recently, Gauthier and coworkers have synthesized a series of 3-O-glycosides of betulinic acid which exhibited a strongly potent in vitro anticancer activity against human cancer cell lines.[14]

[edit] See also

[edit] References

  1. ^ Chowdhury, A. R.; Mandal S., Mittra B., Sharma S., Mukhopadhyay S., Majumder H. K. (July 2002). "Betulinic acid, a potent inhibitor of eukaryotic topoisomerase I: identification of the inhibitory step, the major functional group responsible and development of more potent derivatives". Medical Science Monitor 8 (7): BR254–65. ISSN 1643-3750. 
  2. ^ a b Tan, Y.; Yu, R., Pezzuto J. M. (July 2003). "Betulinic acid-induced programmed cell death in human melanoma cells involves mitogen-activated protein kinase activation". Clinical Cancer Research 9 (7): 2866–75. ISSN 1557-3265. PMID 12855667. 
  3. ^ a b c d e Zuco, V.; Supino R., Righetti S. C., Cleris L., Marchesi E., Gambacorti-Passerini C., Formelli F. (2002-01-10). "Selective cytotoxicity of betulinic acid on tumor cell lines, but not on normal cells". Cancer Letters 175 (1): 17–25. doi:10.1016/S0304-3835(01)00718-2. ISSN 0304-3835. 
  4. ^ Gao, H.; Wu L., Kuroyanagi M., Harada K., Kawahara N., Nakane T., Umehara K., Hirasawa A., Nakamura Y. (November 2003). "Antitumor-promoting constituents from Chaenomeles sinensis KOEHNE and their activities in JB6 mouse epidermal cells". Chemical and Pharmaceutical Bulletin 51 (11): 1318–21. doi:10.1248/cpb.51.1318. ISSN 0009-2363. 
  5. ^ a b Ji, Z. N.; Ye W. C., Liu G. G., Hsiao W. L. (2002-11-22). "23-Hydroxybetulinic acid-mediated apoptosis is accompanied by decreases in bcl-2 expression and telomerase activity in HL-60 Cells". Life Sciences 72 (1): 1–9. doi:10.1016/S0024-3205(02)02176-8. ISSN 0024-3205. 
  6. ^ Pisha, E.; Chai, H., Ik-Soo Lee, Chagwedera, T. E., Farnsworth N. R., Cordell G. A., Beecher C. W., Fong H. H. S., Kinghorn A. D., Brown D. M., Wani M. C., Wall M. E., Hiekens T. J., Das Gupta T. K., Pezzuto J. M. (October 1995). "Discovery of betulinic acid as a selective inhibitor of human melanoma that functions by induction of apoptosis". Nature Medicine 1 (10): 1046–1051. doi:10.1038/nm1095-1046. ISSN 1078-8956. 
  7. ^ Schmidt, M. L.; Kuzmanoff K. L., Ling-Indeck L., Pezzuto J. M. (October 1997). "Betulinic acid induces apoptosis in human neuroblastoma cell lines.". European Journal of Cancer 33 (12): 2007–10. doi:10.1016/S0959-8049(97)00294-3. ISSN 0959-8049. 
  8. ^ a b c d Fulda, S; Friesen C., Los M., Scaffidi C., Mier W., Benedict M., Nunez G., Krammer P. H., Peter M. E., Debatin K. M. (1997-11-01). "Betulinic acid triggers CD95 (APO-1/Fas)- and p53-independent apoptosis via activation of caspases in neuroectodermal tumors". Cancer Research 57 (21): 4956–64. ISSN 0008-5472. PMID 9354463. 
  9. ^ Wick, W.; Grimmel C., Wagenknecht B., Dichgans J., Weller M. (June 1999). "Betulinic acid-induced apoptosis in glioma cells: A sequential requirement for new protein synthesis, formation of reactive oxygen species, and caspase processing". Journal of Pharmacology and Experimental Therapeutics 289 (3): 1306–12. ISSN 0022-3565. PMID 10336521. 
  10. ^ a b c d Thurnher, D. coauthors = Turhani D., Pelzmann M., Wannemacher B., Knerer B., Formanek M., Wacheck V., Selzer E. (September 2003). "Betulinic acid: a new cytotoxic compound against malignant head and neck cancer cells". Head & Neck 25 (9): 732–40. doi:10.1002/hed.10231. ISSN 1043-3074. 
  11. ^ Raisova, M; Hossini A. M., Eberle J., Riebeling C., Wieder T., Sturm I., Daniel P. T., Orfanos C. E., Geilen C. C. (August 2001). "The Bax/Bcl-2 ratio determines the susceptibility of human melanoma cells to CD95/Fas-mediated apoptosis". Journal of Investigative Dermatology 117 (2): 333–40. doi:10.1046/j.0022-202x.2001.01409.x. ISSN 0022-202X. 
  12. ^ a b Rieber, M; Strasberg Rieber M. (May 1998). "Induction of p53 without increase in p21WAF1 in betulinic acid-mediated cell death is preferential for human metastatic melanoma". DNA and Cell Biology 17 (5): 399–406. ISSN 1044-5498. 
  13. ^ a b Selzer, E; Pimentel E., Wacheck V., Schlegel W., Pehamberger H., Jansen B., Kodym R. (May 2000). "Effects of betulinic acid alone and in combination with irradiation in human melanoma cells". Journal Investigative Dermatology 114 (5): 935–40. doi:10.1046/j.1523-1747.2000.00972.x. ISSN 0022-202X. 
  14. ^ Gauthier, C; Legault J, Lebrun M, Dufour P, Pichette A. (2006-10-01). "Glycosidation of lupane-type triterpenoids as potent in vitro cytotoxic agents". Bioorganic and Medicinal Chemistry 14 (19): 6713–25. doi:10.1016/j.bmc.2006.05.075. 
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