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| Bacteriophage pRNA |
 |
| Type: |
Gene; |
| 2° structure: |
Predicted; ILM |
| Seed alignment: |
Finn RD |
| Avg length: |
215.2 nucleotides |
| Avg identity: |
76% |
|
During replication of linear dsDNA viruses, the viral genome is packaged into the pre-formed viral procapsid. The packaging of DNA into the procapsid requires a molecular motor which using ATP as energy to accomplish the energetically unfavourable motion. In some bacteriophage, an RNA (pRNA) molecule is a vital component of this motor [1]. Structural analyses of the packaging motor have demonstrated that the pRNA molecule has fivefold symmetry when attached to the prohead [2][3] [4]. The pRNA is thought to be bound by the capsid connector protein [4]. Only the first 120 bases of the pRNA are essential for packing the viral DNA [5] [6]. The pRNA is proposed to be comprised of two domains, one corresponding to the first 120 bases and the second to the remaining 50 bases [6]. Nuclear cleavage occurs in the single strand region linking these two domains [6].
[edit] References
- ^ Guo P (2002). "Structure and function of phi29 hexameric RNA that drives the viral DNA packaging motor: review". Prog. Nucleic Acid Res. Mol. Biol. 72: 415–72. PMID 12206459.
- ^ Morais MC, Tao Y, Olson NH, et al (2001). "Cryoelectron-microscopy image reconstruction of symmetry mismatches in bacteriophage phi29". J. Struct. Biol. 135 (1): 38–46. doi:10.1006/jsbi.2001.4379. PMID 11562164.
- ^ Simpson AA, Tao Y, Leiman PG, et al (2000). "Structure of the bacteriophage phi29 DNA packaging motor". Nature 408 (6813): 745–50. doi:10.1038/35047129. PMID 11130079.
- ^ a b Badasso MO, Leiman PG, Tao Y, et al (2000). "Purification, crystallization and initial X-ray analysis of the head-tail connector of bacteriophage phi29". Acta Crystallogr. D Biol. Crystallogr. 56 (Pt 9): 1187–90. PMID 10957642.
- ^ Guo PX, Erickson S, Anderson D (1987). "A small viral RNA is required for in vitro packaging of bacteriophage phi 29 DNA". Science 236 (4802): 690–4. PMID 3107124.
- ^ a b c Bailey S, Wichitwechkarn J, Johnson D, Reilly BE, Anderson DL, Bodley JW (1990). "Phylogenetic analysis and secondary structure of the Bacillus subtilis bacteriophage RNA required for DNA packaging". J. Biol. Chem. 265 (36): 22365–70. PMID 2125049.
[edit] External links
