Bacillus stearothermophilus
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| Geobacillus stearothermophilus | ||||||||||||||
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| Bacillus stearothermophilus |
Bacillus stearothermophilus (or Geobacillus stearothermophilus)[1] is a rod-shaped, Gram-positive bacterium and a member of the division Firmicutes. The bacteria is a thermophile and is widely distributed in soil, hot springs, ocean sediment, and is a cause of spoilage in food products. It is commonly used as a challenge organism for sterilization validation studies and periodic check of sterilization cycles. The biological indicator contains spores of the organism on filter paper inside a vial. After sterilizing the cap is closed, an ampule of growth medium inside of the vial is crushed and the whole vial is incubated. A color and/or turbidity change indicates the results of the sterilization process, no change in indicates sterilization conditions were achieved, otherwise the growth of the spores indicates that the sterilization process has not been met.
Examples of this type of Biological Indicator (BI) using this bacillus are Getinge's Biosign Steam-24 .
(BI)'s are used in conjunction with Chemical Indicators (CI) and Process Indicators (PI) to validate sterilization processes.
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