Auramine O
From Wikipedia, the free encyclopedia
| Auramine O | |
|---|---|
 |
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| IUPAC name | bis[4-(dimethylamino)phenyl]methaniminium chloride |
| Identifiers | |
| CAS number | [2465-27-2] |
| SMILES | [NH2+]=C (C2=CC=C (N(C)C)C=C2) C1=CC=C(N (C)C)C=C1 |
| Properties | |
| Molecular formula | C17H22N3Cl |
| Molar mass | 303.8339 g/mol |
| Melting point |
267 °C |
| Hazards | |
| R-phrases | R22 R24 R40 |
| S-phrases | S36/37 S45 |
| Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) Infobox disclaimer and references |
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Auramine O, also called Basic yellow 2, Pyocatanium aureum, aizen auramine, Pyoktanin Yellow, Canary Yellow, Pyoktanin, or C.I. 41000, is a diarylmethane dye used as a fluorescent stain. In its pure form, Auramine O appears as yellow needle crystals. It is very soluble in water and soluble in ethanol.
Auramine O can be used to stain acid-fast bacteria (e.g. Mycobacterium), where it binds to the mycolic acid in its cell wall) in a way similar to Ziehl-Neelsen stain.[1] It can also be used as a fluorescent version of Schiff reagent.[2]
Auramine O can be used together with Rhodamine B as the Truant auramine-rhodamine stain for Mycobacterium tuberculosis.[3][4] It can be also used as an antiseptic agent.
[edit] References
- ^ Kommareddi S, Abramowsky C, Swinehart G, Hrabak L (1984). "Nontuberculous mycobacterial infections: comparison of the fluorescent auramine-O and Ziehl-Neelsen techniques in tissue diagnosis". Hum Pathol 15 (11): 1085–9. doi:. PMID 6208117.
- ^ Khavkin T, Kudryavtseva M, Dragunskaya E, Polotsky Y, Kudryavtsev B (1980). "Fluorescent PAS-reaction study of the epithelium of normal rabbit ileum and after challenge with enterotoxigenic Escherichia coli". Gastroenterology 78 (4): 782–90. PMID 6986320.
- ^ Truant J, Brett W, Thomas W (1962). "Fluorescence microscopy of tubercle bacilli stained with auramine and rhodamine". Henry Ford Hosp Med Bull 10: 287–96. PMID 13922644.
- ^ Arrowood M, Sterling C (1989). "Comparison of conventional staining methods and monoclonal antibody-based methods for Cryptosporidium oocyst detection". J Clin Microbiol 27 (7): 1490–5. PMID 2475523.
