Aquacobalamin reductase (NADPH)

From Wikipedia, the free encyclopedia

In enzymology, an aquacobalamin reductase (NADPH) (EC 1.16.1.5) is an enzyme that catalyzes the chemical reaction

2 cob(II)alamin + NADP⁺ $\rightleftharpoons$ 2 aquacob(III)alamin + NADPH + H⁺

Thus, the two substrates of this enzyme are cob(II)alamin and NADP⁺, whereas its 3 products are aquacob(III)alamin, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those oxidizing metal ion with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is cob(II)alamin:NADP+ oxidoreductase. Other names in common use include aquacobalamin (reduced nicotinamide adenine dinucleotide phosphate), reductase, NADPH-linked aquacobalamin reductase, and NADPH2:aquacob(III)alamin oxidoreductase. This enzyme participates in porphyrin and chlorophyll metabolism. It employs one cofactor, FAD.

[edit] References

IUBMB entry for 1.16.1.5
BRENDA references for 1.16.1.5 (Recommended.)
PubMed references for 1.16.1.5
PubMed Central references for 1.16.1.5
Google Scholar references for 1.16.1.5
Watanabe F, Oki Y, Nakano Y, Kitaoka S (1987). "Purification and characterization of aquacobalamin reductase (NADPH) from Euglena gracilis". J. Biol. Chem. 262: 11514–8. PMID 3114247.
Watanabe F, Yamaji R, Isegawa Y, Yamamoto T, Tamura Y, Nakano Y (1993). "Characterization of aquacobalamin reductase (NADPH) from Euglena gracilis". Arch. Biochem. Biophys. 305: 421–7. doi:10.1006/abbi.1993.1441. PMID 8373179.