Amido black
From Wikipedia, the free encyclopedia
| Amido black | |
|---|---|
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| IUPAC name | 4-Amino-5-hydroxy-3-[(4-nitrophenyl)azo]-6- -(phenylazo)-2,7-Naphthalene disulfonic acid, disodium salt |
| Identifiers | |
| CAS number | [1064-48-8] |
| PubChem | |
| SMILES | OC1=C(C(N)=C(/N=N/C4=CC=C([N+]([O-])=O) C=C4)C(S(=O)(O[Na])=O)=C3)C3=CC(S(=O) (O[Na])=O)=C1/N=N/C2=CC=CC=C2 |
| Properties | |
| Molecular formula | C22H14N6Na2O9S2 |
| Molar mass | 616.491 |
| Appearance | Dark red to black |
| Solubility in water | ~ 30 g/l at 20 °C |
| Hazards | |
| NFPA 704 |
 0
2
0
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| R-phrases | R20, R21, R33 |
| S-phrases | S28A, S37, S45 |
| Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) Infobox disclaimer and references |
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Amido black 10B, also called Amidoschwarz, Naphthol blue black, Acid Black 1, Acidal Black 10B , Acidal Navy Blue 3BR, Naphthalene Black 10B, Buffalo Black NBR and C.I. 20470, is an amino acid staining diazo dye used in biochemical research to stain for total protein on transferred membrane blots. It is also used in criminal investigations to detect blood present with latent fingerprints. It stains the proteins in blood a blue-black color. Amido Black can be either methanol or water based as it readily dissolves in both. With picric acid, in a van Gieson procedure, it can be used to stain collagen and reticulin.
