Aerobactin synthase

From Wikipedia, the free encyclopedia

In enzymology, an aerobactin synthase (EC 6.3.2.27) is an enzyme that catalyzes the chemical reaction

4 ATP + citrate + 2 N₆-acetyl-N₆-hydroxy-L-lysine + 2 H₂O $\rightleftharpoons$ 4 ADP + 4 phosphate + aerobactin

The 4 substrates of this enzyme are ATP, citrate, N6-acetyl-N6-hydroxy-L-lysine, and H₂O, whereas its 3 products are ADP, phosphate, and aerobactin.

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds as acid-D-amino-acid ligases (peptide synthases). The systematic name of this enzyme class is citrate:N6-acetyl-N6-hydroxy-L-lysine ligase (ADP-forming). This enzyme is also called citrate:6-N-acetyl-6-N-hydroxy-L-lysine ligase (ADP-forming). This enzyme participates in lysine degradation.

[edit] References

IUBMB entry for 6.3.2.27
BRENDA references for 6.3.2.27 (Recommended.)
PubMed references for 6.3.2.27
PubMed Central references for 6.3.2.27
Google Scholar references for 6.3.2.27
Appanna DL, Grundy BJ, Szczepan EW and Viswanatha T (1984). "Aerobactin synthesis in a cell-free system of Aerobacter aerogenes 62-1". Biochim. Biophys. Acta 801: 437–443.
Gibson F, Magrath DI (1969). "The isolation and characterization of a hydroxamic acid (aerobactin) formed by Aerobacter aerogenes 62-I". Biochim. Biophys. Acta. 192: 175–84. PMID 4313071.
Maurer PJ and Miller M (1982). "Microbial iron chelators: total synthesis of aerobactin and its constituent amino acid, N6-acetyl-N6-hydroxylysine". J. Am. Chem. Soc. 104: 3096–3101. doi:10.1021/ja00375a025.
de Lorenzo V, Bindereif A, Paw BH, Neilands JB (1986). "Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12". J. Bacteriol. 165: 570–8. PMID 2935523.
Challis GL (2005). "A widely distributed bacterial pathway for siderophore biosynthesis independent of nonribosomal peptide synthetases". Chembiochem. 6: 601–11. doi:10.1002/cbic.200400283. PMID 15719346.