8-dimethylallylnaringenin 2'-hydroxylase

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In enzymology, a 8-dimethylallylnaringenin 2'-hydroxylase (EC 1.14.13.103) is an enzyme that catalyzes the chemical reaction

sophoraflavanone B + NADPH + H⁺ + O₂ $\rightleftharpoons$ leachianone G + NADP⁺ + H₂O

The 4 substrates of this enzyme are sophoraflavanone B, NADPH, H⁺, and O₂, whereas its 3 products are leachianone G, NADP⁺, and H₂O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor. The systematic name of this enzyme class is sophoraflavanone-B,NADPH:oxygen oxidoreductase (2'-hydroxylating). This enzyme is also called 8-DMAN 2'-hydroxylase.

[edit] References

IUBMB entry for 1.14.13.103
BRENDA references for 1.14.13.103 (Recommended.)
PubMed references for 1.14.13.103
PubMed Central references for 1.14.13.103
Google Scholar references for 1.14.13.103
Yamamoto H, Yatou A, Inoue K (2001). "8-dimethylallylnaringenin 2'-hydroxylase, the crucial cytochrome P450 mono-oxygenase for lavandulylated flavanone formation in Sophora flavescens cultured cells". Phytochemistry. 58: 671–6. doi:10.1016/S0031-9422(01)00270-9. PMID 11672730.
Zhao P, Inoue K, Kouno I, Yamamoto H (2003). "Characterization of leachianone G 2"-dimethylallyltransferase, a novel prenyl side-chain elongation enzyme for the formation of the lavandulyl group of sophoraflavanone G in Sophora flavescens Ait cell suspension cultures". Plant. Physiol. 133: 1306–13. doi:10.1104/pp.103.025213. PMID 14551337.