7alpha-hydroxysteroid dehydrogenase

From Wikipedia, the free encyclopedia

In enzymology, a 7alpha-hydroxysteroid dehydrogenase (EC 1.1.1.159) is an enzyme that catalyzes the chemical reaction

3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + NAD⁺ $\rightleftharpoons$ 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H⁺

Thus, the two substrates of this enzyme are 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate and NAD⁺, whereas its 3 products are 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate, NADH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 7alpha-hydroxysteroid:NAD+ 7-oxidoreductase. Other names in common use include 7alpha-hydroxy steroid dehydrogenase, and 7alpha-HSDH.

1 Structural studies
2 References
3 External links
- 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 1AHH, 1AHI, and 1FMC.

[edit] References

IUBMB entry for 1.1.1.159
BRENDA references for 1.1.1.159 (Recommended.)
PubMed references for 1.1.1.159
PubMed Central references for 1.1.1.159
Google Scholar references for 1.1.1.159
Haslewood ES, Haslewood GA (1976). "The specificity of a 7 alpha-hydroxy steroid dehydrogenase from Escherichia coli". Biochem. J. 157: 207–10. PMID 786279.
MacDonald IA, Roach PD (1981). "Bile induction of 7 alpha- and 7 beta-hydroxysteroid dehydrogenases in Clostridium absonum". Biochim. Biophys. Acta. 665: 262–9. PMID 6945134.
Macdonald IA, Williams CN, Mahony DE (1973). "7Alpha-hydroxysteroid dehydrogenase from Escherichia coli B: preliminary studies". Biochim. Biophys. Acta. 309: 243–53. PMID 4581498.
Macdonald IA, Williams CN, Mahony DE, Christie WM (1975). "NAD- and NADP-dependent 7alpha-hydroxysteroid dehydrogenases from bacteroides fragilis". Biochim. Biophys. Acta. 384: 12–24. PMID 236764.