5S ribosomal RNA

5S ribosomal RNA

Type:	Gene; rRNA;
2° structure:	Published^[1]
Seed alignment:	Szymanski et al, 5S ribosomal database^[2]
Avg length:	116.8 nucleotides
Avg identity:	61%

5S ribosomal RNA (5S rRNA) is a component of the large ribosomal subunit in both prokaryotes and eukaryotes.

In prokaryotes, it is part of the 50S subunit.
In eukaryotes, it is synthesised by RNA polymerase III (the other eukaryotic rRNAs are cleaved from a 45S precursor synthesized by RNA polymerase I). In Xenopus oocytes, it has been shown that fingers 4-7 of the nine-zinc finger transcription factor TFIIIA can bind to the central region of 5S RNA.^[3] Binding between 5S rRNA and TFIIIA serves to both repress further transcription of the 5S RNA gene and stabilize the 5S RNA transcript until it is required for ribosome assembly.^[4]

[edit] References

^ Yusupov, MM; Yusupova GZ, Baucom A, Lieberman K, Earnest TN, Cate JH, Noller HF (2001). "Crystal structure of the ribosome at 5.5 A resolution". Science 292: 883–896. doi:10.1126/science.1060089. PMID 11283358.
^ Szymanski, M; Barciszewska MZ, Erdmann VA, Barciszewski J (2002). "5S Ribosomal RNA Database". Nucleic Acids Res 30: 176–178. doi:10.1093/nar/30.1.176. PMID 11752286.
^ Searles, MA; Lu D, Klug A (2000). "The role of the central zinc fingers of transcription factor IIIA in binding to 5 S RNA". J Mol Biol 301: 47–60. doi:10.1006/jmbi.2000.3946. PMID 10926492.
^ Pelham, HRB; Brown DD (1980). "A specific transcription factor that can bind either the 5S RNA gene or 5S RNA". Proc Natl Acad Sci 77: 4170–4174. doi:10.1073/pnas.77.7.4170. PMID 7001457.