4-methoxybenzoate monooxygenase (O-demethylating)

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In enzymology, a 4-methoxybenzoate monooxygenase (O-demethylating) (EC 1.14.99.15) is an enzyme that catalyzes the chemical reaction

4-methoxybenzoate + AH₂ + O₂ $\rightleftharpoons$ 4-hydroxybenzoate + formaldehyde + A + H₂O

The 3 substrates of this enzyme are 4-methoxybenzoate, AH2, and O₂, whereas its 4 products are 4-hydroxybenzoate, formaldehyde, A, and H₂O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derive from O miscellaneous. The systematic name of this enzyme class is 4-methoxybenzoate,hydrogen-donor:oxygen oxidoreductase (O-demethylating). Other names in common use include 4-methoxybenzoate 4-monooxygenase (O-demethylating), 4-methoxybenzoate O-demethylase, p-anisic O-demethylase, and piperonylate-4-O-demethylase. This enzyme participates in 2,4-dichlorobenzoate degradation.

[edit] References

IUBMB entry for 1.14.99.15
BRENDA references for 1.14.99.15 (Recommended.)
PubMed references for 1.14.99.15
PubMed Central references for 1.14.99.15
Google Scholar references for 1.14.99.15
Bernhardt FH, Nastainczyk W, Seydewitz V (1977). "Kinetic studies on a 4-methoxybenzoate O-demethylase from Pseudomonas putida". Eur. J. Biochem. 72: 107–15. PMID 188654.
Paszczynski A, Trojanowski J (1977). "An affinity-column procedure for the purification of veratrate O-demethylase from fungi". Microbios. 18: 111–21. PMID 25369.
Twilfer H, Bernhardt FH, Gersonde K (1981). "An electron-spin-resonance study on the redox-active centers of the 4-methoxybenzoate monooxygenase from Pseudomonas putida". Eur. J. Biochem. 119: 595–602. PMID 6273164.