3alpha,7alpha,12alpha-trihydroxycholestan-26-al 26-oxidoreductase

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In enzymology, a 3alpha,7alpha,12alpha-trihydroxycholestan-26-al 26-oxidoreductase (EC 1.2.1.40) is an enzyme that catalyzes the chemical reaction

(25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-al + NAD⁺ + H₂O $\rightleftharpoons$ (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate + NADH + 2 H⁺

The 3 substrates of this enzyme are (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-al, NAD⁺, and H₂O, whereas its 3 products are (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate, NADH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-al:NAD+ 26-oxidoreductase. Other names in common use include cholestanetriol-26-al 26-dehydrogenase, 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-al, dehydrogenase, trihydroxydeoxycoprostanal dehydrogenase, THAL-NAD oxidoreductase, 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-al:NAD+, and 26-oxidoreductase. This enzyme participates in bile acid biosynthesis.

[edit] References

IUBMB entry for 1.2.1.40
BRENDA references for 1.2.1.40 (Recommended.)
PubMed references for 1.2.1.40
PubMed Central references for 1.2.1.40
Google Scholar references for 1.2.1.40
Masui T, Herman R, Staple E (1966). "The oxidation of 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha, 26-tetraol to 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol-26-oic acid via 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol-26-al by rat liver". Biochim. Biophys. Acta. 117: 266–8. PMID 5914340.