3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase

From Wikipedia, the free encyclopedia

In enzymology, a 3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase (EC 4.2.1.107) is an enzyme that catalyzes the chemical reaction

(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA $\rightleftharpoons$ (24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA + H₂O

Thus, the substrate of this enzyme are (24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA, whereas its two products are (24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA and H₂O.

This enzyme belongs to the family of lyases, specifically the hydro-lyases, which cleave carbon-oxygen bonds. The systematic name of this enzyme class is (24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA hydro-lyase [(24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA-forming]. Other names in common use include 46 kDa hydratase 2, and (24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA hydro-lyase.

[edit] References

IUBMB entry for 4.2.1.107
BRENDA references for 4.2.1.107 (Recommended.)
PubMed references for 4.2.1.107
PubMed Central references for 4.2.1.107
Google Scholar references for 4.2.1.107
Qin YM, Haapalainen AM, Conry D, Cuebas DA, Hiltunen JK, Novikov DK (Pt 2). "Recombinant 2-enoyl-CoA hydratase derived from rat peroxisomal multifunctional enzyme 2: role of the hydratase reaction in bile acid synthesis". Biochem. J. 328: 377–82. PMID 9371691.
Xu R, Cuebas DA (1996). "The reactions catalyzed by the inducible bifunctional enzyme of rat liver peroxisomes cannot lead to the formation of bile acids". Biochem. Biophys. Res. Commun. 221: 271–8. doi:10.1006/bbrc.1996.0585. PMID 8619845.
Kokawa NI and Morisaki M (1988). "Synthesis and determination of stereochemistry of four diastereoisomers at the C-24 and C-25 positions of 3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestan-26-oic acid and cholic acid". Chem. Pharm. Bull. 36: 134–141.
Sonoda Y, Sato Y and Morisaki M (1988). "Non-stereoselective conversion of the four diastereoisomers at the C-24 and C-25 positions of 3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestan-26-oic acid and cholic acid". Chem. Pharm. Bull. 36: 142–145.
Hashimoto T (2001). "Conjugation reactions catalyzed by bifunctional proteins related to beta-oxidation in bile acid biosynthesis". Steroids. 66: 107–14. doi:10.1016/S0039-128X(00)00217-8. PMID 11146090.
Russell DW (2003). "The enzymes, regulation, and genetics of bile acid synthesis". Annu. Rev. Biochem. 72: 137–74. doi:10.1146/annurev.biochem.72.121801.161712. PMID 12543708.