3,5-Dinitrosalicylic acid

From Wikipedia, the free encyclopedia

3,5-Dinitrosalicylic acid (DNS) is an aromatic compound that reacts with reducing sugars and other reducing molecules to form 3-amino-5-nitrosalicylic acid, which absorbs light strongly at 540 nm. It was first introduced as a method to detect reducing substances in urine and has since been widely used, for example, for quantificating carbohydrates levels in blood. It is mainly used in assay of alpha-amylase. However, enzymatic methods are usually preferred to DNS due to their specificity.

[edit] External links

• Description of lab use from the Department of Chemical Engineering, University of Maryland