27-hydroxycholesterol 7alpha-monooxygenase

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In enzymology, a 27-hydroxycholesterol 7alpha-monooxygenase (EC 1.14.13.60) is an enzyme that catalyzes the chemical reaction

27-hydroxycholesterol + NADPH + H⁺ + O₂ 7alpha,27-dihydroxycholesterol + NADP⁺ + H₂O

The 4 substrates of this enzyme are 27-hydroxycholesterol, NADPH, H⁺, and O₂, whereas its 3 products are 7alpha,27-dihydroxycholesterol, NADP⁺, and H₂O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor. The systematic name of this enzyme class is 27-hydroxycholesterol,NADPH:oxygen oxidoreductase (7alpha-hydroxylating). This enzyme is also called 27-hydroxycholesterol 7alpha-hydroxylase. It employs one cofactor, heme-thiolate(P-450).

[edit] References

• IUBMB entry for 1.14.13.60
• BRENDA references for 1.14.13.60 (Recommended.)
• PubMed references for 1.14.13.60
• PubMed Central references for 1.14.13.60
• Google Scholar references for 1.14.13.60
• Kumiko OM, Budai K and Javitt NB (1993). "Cholesterol and 27-hydroxycholesterol 7alpha-hydroxylation: evidence for two different enzymes". J. Lipid Res. 34: 581–588. 

[edit] External links

The CAS registry number for this enzyme class is 149316-80-3.

• IUBMB entry for 1.14.13.60

• KEGG entry for 1.14.13.60

• BRENDA entry for 1.14.13.60

• NiceZyme view of 1.14.13.60

• EC2PDB: PDB structures for 1.14.13.60

• PRIAM entry for 1.14.13.60

• PUMA2 entry for 1.14.13.60

• IntEnz: Integrated Enzyme entry for 1.14.13.60

• MetaCyc entry for 1.14.13.60

• Atomic-resolution structures of enzymes belonging to this class

[edit] Gene Ontology (GO) codes

• EGO entry for GO code 0047092: 27-hydroxycholesterol 7-alpha-monooxygenase

• AMIGO entry for GO code 0047092: 27-hydroxycholesterol 7-alpha-monooxygenase