2'-phosphotransferase

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In enzymology, a 2'-phosphotransferase (EC 2.7.1.160) is an enzyme that catalyzes the chemical reaction

2'-phospho-[ligated tRNA] + NAD⁺ $\rightleftharpoons$ mature tRNA + ADP-ribose 1,2-phosphate + nicotinamide + H₂O

Thus, the two substrates of this enzyme are [[2'-phospho-[ligated tRNA]]] and NAD⁺, whereas its 4 products are mature tRNA, [[ADP-ribose 1,2-phosphate]], nicotinamide, and H₂O.

This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The systematic name of this enzyme class is 2'-phospho-[ligated tRNA]:NAD+ phosphotransferase. Other names in common use include yeast 2'-phosphotransferase, Tpt1, Tpt1p, and 2'-phospho-tRNA:NAD+ phosphotransferase.

[edit] References

IUBMB entry for 2.7.1.160
BRENDA references for 2.7.1.160 (Recommended.)
PubMed references for 2.7.1.160
PubMed Central references for 2.7.1.160
Google Scholar references for 2.7.1.160
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Spinelli SL, Kierzek R, Turner DH, Phizicky EM (1999). "Transient ADP-ribosylation of a 2'-phosphate implicated in its removal from ligated tRNA during splicing in yeast". J. Biol. Chem. 274: 2637–44. doi:10.1074/jbc.274.5.2637. PMID 9915792.
Culver GM, McCraith SM, Consaul SA, Stanford DR, Phizicky EM (1997). "A 2'-phosphotransferase implicated in tRNA splicing is essential in Saccharomyces cerevisiae". J. Biol. Chem. 272: 13203–10. doi:10.1074/jbc.272.20.13203. PMID 9148937.
McCraith SM, Phizicky EM (1991). "An enzyme from Saccharomyces cerevisiae uses NAD+ to transfer the splice junction 2'-phosphate from ligated tRNA to an acceptor molecule". J. Biol. Chem. 266: 11986–92. PMID 2050693.
Hu QD, Lu H, Huo K, Ying K, Li J, Xie Y, Mao Y, Li YY (2003). "A human homolog of the yeast gene encoding tRNA 2'-phosphotransferase: cloning, characterization and complementation analysis". Cell. Mol. Life. Sci. 60: 1725–32. doi:10.1007/s00018-003-3107-7. PMID 14504659.
Steiger MA, Jackman JE, Phizicky EM (2005). "Analysis of 2'-phosphotransferase (Tpt1p) from Saccharomyces cerevisiae: evidence for a conserved two-step reaction mechanism". RNA. 11: 99–106. doi:10.1261/rna.7194605. PMID 15611300.
Sawaya R, Schwer B, Shuman S (2005). "Structure-function analysis of the yeast NAD+-dependent tRNA 2'-phosphotransferase Tpt1". RNA. 11: 107–13. doi:10.1261/rna.7193705. PMID 15611301.
Kato-Murayama M, Bessho Y, Shirouzu M, Yokoyama S (2005). "Crystal structure of the RNA 2'-phosphotransferase from Aeropyrum pernix K1". J. Mol. Biol. 348: 295–305. doi:10.1016/j.jmb.2005.02.049. PMID 15811369.