Talk:Transcription (genetics)
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[edit] Harvard Papers?
"By the late 1960s several papers that came out of the Harvard University Biological Laboratories established the basic mechanics of gene expression in bacteria."
This information is not related to this article in context and will be deleted. If you're going to include this you might as well include all the articles on pub med because the "basic mechanics of gene expression in bacteria" is a communitative effort not just exclusivly by faculty in Harvard. Although I presume they had made their contributions as well. It's just not correct to include one place that contributed to gene expression, so I assume the person who included this is maybe from the school itself.
[edit] Reverse transcriptase
According to the current article, transcription refers solely to DNA>RNA transcription, and even goes to far as to link to Crick's central dogma (DNA is transcribed to RNA which is translated to polypeptides, never the other way around). Someone should make it clear from the start that transcription, while usually used to refer to DNA>RNA transcription, might also refer to RNA>DNA transcription. -- Ec5618 23:39, 4 December 2005 (UTC)
I added a beginning of informations plus a little scheme I made earlier ... I think it should be in another page, but I don't know how to do ;-P. Hope someone will ! -- Totophe64
[edit] Eukaryotic Transcription
Edit 4/2/06 explanation Most eukaryotes have 4 RNA polymerases, plants have 5. Although RNAP is a commonly used abbreviation for bacterial and bacteriophage RNA polymerases, it is almost never used for eukaryotic RNA polymerases. I have substituted Pol I, II and III. (This will also need to be done in the subsections.) I have also included Pol A, B, and C not only in recognition of the work of Pierre Chambon and Andre Sentenac (vs. the nomenclature of William Rutter and Robert Roeder), but at some point the gene names (eg., RPA1, RPB1, RPC1) will come up as this section expands, and this edit will help clarify this nomenclature. -opus118
Organization and additional items needed "Perhaps one way of organizing this section is to group the different RNA polymerases: Archaea, bacteria, chloroplast, and eukaryotic nuclear RNA polymerases form one related group. Bacteriophage T7-like and mitochondrial RNA polymerases form a second, DNA polymerase I-derived group (does reverse transcriptase fall into this category?), and then a diverse mixture of bacteriophage and viral RNA polymerases that do not fall within the above two categories. There should be some emphasis on common mechanisms and related subunits. For example: 1) the β'βα2ω subunits of bacteral RNA polymerase have sequence-related homologs in chloroplast RNA polymerase, archaeal RNA polymerase and Pol I, II and III. 2) The 12 (?) subunits of archaeal RNA polymerase have homologs in Pol I, II and III (with Pol III containing 5 additional unique subunits); 3) TBP is a transcription factor for archaeal, Pol I, Pol II, and Pol III transcription. 4) A TFIIB-related factor is common for archaeal, Pol II and pol III transcription. 5)TFIIH functions for Pol I and II transcription. 6) Promoter opening occurs with an upstream to downstream polarity with all polymerases. 7) Stem-loop structures at sites of termination are common (with the possible exception of Pol III). 8) The mechanism of catalysis for RNA chain elongation is identical (the active site region is highly conserved). 9) bacterial GreA/GreB, TFIIS and Rpc11 all stimulate a 3' to 5' ribonucleolytic activity at the RNA polymerase active site by inserting a finger-like structure in the nucleotide substrate feeding pore of RNA polymerase to place a pair of acidic residues near the catalytic site that chelates an additional Mg2+ ion for hydrolysis. It is the polymerase-unique properties that might be best dealt with in subsections. -opus18
[edit] Termination
- Garbeled section needs Editing. Something missing or duplicated etc in the "sentence(s)":
- in the DNA template. or where a GC-rich inverted
- 4 A residues. the inverted repeat forms
- dissociate from the DNA template. where the -35 region
- See the following surround section.
*Rho-dependent termination uses a termination factor called ρ factor(rho factor) to stop RNA synthesis at specific sites. This protein binds and runs along the mRNA towards the RNAP. When ρ-factor reaches the RNAP, it causes RNAP to dissociate from the DNA, terminating transcription. Other termination mechanisms include where RNAP comes across a region with repetitious thymidine residues in the DNA template. or where a GC-rich inverted repeat followed by 4 A residues. the inverted repeat forms a stable stem loop structure in the Rna, which causes the RNA to dissociate from the DNA template. where the -35 region and the -10 ("Pribnow box") region comprise the basic prokaryotic promoter, and |T| stands for the terminator. The DNA on the template strand between the +1 site and the terminator is transcribed into RNA, which is then translated into protein.
DLH 17:37, 10 September 2006 (UTC)
[edit] category
Surely this article should be under Molecular Biology? answer: WHY? Where else should it be?
[edit] order?
Is there any reason why it shows "see also" and "external links" then more of the article, then another "external links" section? If I dont' get any replies within a week or so, I'll go ahead and move the rest of the article up, and combine the two "external links" sections. jf 22:20, 23 May 2006 (UTC)
[edit] Other
"For instance, in eukaryotes the genetic material (DNA), and therefore transcription, is primarily localized to the nucleus, where it is separated from the cytoplasm (where translation occurs) by the nuclear membrane." This sentence has a very unclear structure*.
[edit] I miss the pictures!!!
YES! I do!!!!!!! Somebody, add some, please, it would be VERY IMPORTANT!!! Myrmeleon formicarius 12:42, 12 March 2007 (UTC)
