TBE Buffer
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TBE or Tris/Borate/EDTA, is a buffer solution that consists of a mixture of Tris base, boric acid, EDTA, and water.
In molecular biology, TBE and TAE are often used in procedures involving DNA, the most common being electrophoresis. Tris is an effective buffer for slightly basic solutions, which keeps DNA deprotonated and soluble in water. EDTA binds to divalent cations, particularly magnesium (Mg2+). These ions are necessary co-factors for many enzymes; Magnesium is a co-factor for many DNA-modifying enzymes.
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