User talk:Splette

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[edit] Welcome message

Welcome!

Hello, Splette, and welcome to Wikipedia! Thank you for your contributions. I hope you like the place and decide to stay. Here are a few good links for newcomers:

I hope you enjoy editing here and being a Wikipedian! Please sign your name on talk pages using four tildes (~~~~); this will automatically produce your name and the date. If you need help, check out Wikipedia:Where to ask a question, ask me on my talk page, or place {{helpme}} on your talk page and someone will show up shortly to answer your questions. Again, welcome!

[edit] Copyvio issue

I saw your message at the talk page of Service Civil International. I need evidence of your claim of permission; please note that publishing this page here means you release the text under the GFDL. Send an e-mail to permissions at wikimedia dot org, mentioning this page. Thank you. Chick Bowen 02:35, 18 February 2006 (UTC)

[edit] PyMOL

I strongly disagree with your assertion that PyMOL has "nothing to do with chemistry". It is a molecular visualisation tool and is used by chemists and biochemists for molecules of all sizes from quite small, which is my use, to large proteins. Why did you assert that? I would be interested to know before I think about reverting your last edit. --Bduke 21:44, 24 February 2006 (UTC)

Ok, if you insist, revert my deletion. From my knowledge Pymol is mostly used for representation of proteins and DNA and rather seldom for other chemical components... --Splette 22:05, 24 February 2006 (UTC)

I guess it is used quite a lot in the area between small molecules and proteins or DNA. I have been using it for some drugs, which clearly are chemistry. Of course it could be argued that DNA is chemistry too, but I will not go down that road. The last think we want is a disagreement between chemists and biochemists (there are quite enough wars on WP!), so if in doubt I think we should use both stubs. --Bduke 22:58, 24 February 2006 (UTC)

Thats fine with me. I thought someone who doesn't know what he was doing put that tag there ages ago. Seems like I was wrong. - Splette 15:47, 26 February 2006 (UTC)

[edit] Übersetzung

Hallo Splette,
danke erstmal für deine Übersetzung von Jordanhill Railway Station. Wenn du mal wieder was übersetzt, denke bitte daran, die ursprünglichen Autoren zu würdigen (wie es die GNU-FDL vorschreibt). Ich habe das mal für dich nachgeholt, aber es wäre besser gewesen, dass bereits in der 1ten Version zu vermerken.
Falls du mir antworten möchtest, so hinterlasse mir bitte einen Kommentar auf meiner deutschsprachigen Disku, ich bin zu selten in en: --DaB.

Hi DaB., danke fuer den Hinweis. Das war mir gar nicht klar, dass ich nicht nur beim Einbauen von externen Texten darauf achten muss, sondern auch Wikipedia intern. Macht aber irgendwo Sinn. Ich werde in Zukunft darauf achten. Es sollte aber mal jemand den Artikel weiter uebersetzen. Da ist in den letzten Stunden ja einiges an Inhalt hinzugekommen...

--Splette 14:47, 2 March 2006 (UTC)

[edit] PyMOL copyright on pictures

That's difficult question, but I think it comes down to does an Adobe Illustrator have copyright over figures that I make with it? Secondly, I don't think DeLano would care if we emailed them. We do cite them by linking to PyMOL if click on the image. Let me know what you think. --vossman 23:57, 2 March 2006 (UTC)

Hi Neil, I am not a license expert but the source code of PyMol is licensed under the GNU General Public License (GPL). Thus I was wondering if the created images of this free software have to have be shared under the same license or if they in fact are public domain. I couldn't find anything regarding this topic in their license agreement. I really don't know but it would be good to find out. I think in the future more and more pictures of proteins or chemical compounds will be rendered with PyMol and published here.

Well, one thing is for sure. When creating an image there has to be a citation in any case: "All non-sponsors who use this Build to prepare a publication, presentation, animation, or web site must therein acknowledge PyMOL by name. Failure to do so creates a spnsorship obligation in the form of a liability for payment of current-year PyMOL subscription fees to DeLano Scientific LLC." --Splette

18:53, 3 March 2006 (UTC)

Well, I am a sponsor of the project so I have no worries. :) I agree with you and I will release all future pictures under the GPL. For my journal publication I do reference them, but most of those pictures don't belong here on Wikipedia (too specialized). I'll try to contact DeLano Scientific for their feelings on Wikipedia specifically. --vossman 20:46, 4 March 2006 (UTC)

[edit] My RfA

Hey Splette, how is it going? Thank you for supporting my Request for adminship! It passed with a final vote of 73/1/1, which means that I have been granted adminship! I look forward to using these tools to enhance and maintain this wonderful site. I will continue regular article/project contributions, but I will also allocate a sizable portion of my wikischedule toward administrative duties :) Thanks again, and if you have any questions/comments/tips, please let me know! — Deckiller 04:40, 10 March 2006 (UTC)

Hey, I'm not sure how to communicate with people on this. I think you got the Arp2/3 slightly wrong. I think it should say that in the barbed end branching model arp2/3 associates with the pointed end.

amulekii —The preceding unsigned comment was added by amulekii (talk • contribs).

How can I talk to you? I'm trying to figure out how to nucleate some actin growth. Do I need a WASP to activate my arp2/3 if I wanted to use it to nucleate? Are there other ways to nucleate it that might not require such a two-step process? Amulekii 21:40, 18 May 2006 (UTC)

I guess while I'm at it, I wanted to know how I could stabilize actin. Does phalloidin do a good job? Is it toxic? Is there a cheap way to stabilize actin without phalloidin (like some peptide I could synthesize)? Thanks. Amulekii 22:11, 18 May 2006 (UTC)

Hey, is this where I'm supposed to talk to you? I don't really get this wikipedia thing.

Yes, I'm in Ph.D., but I had to start over after I proved to my professor that what he wanted mt to do was impossible. He wants me to make monodisperse protein-based filaments. I wanted to use actin or MTs maybe (or any other natural filament). I wanted to be able to nucleate so that I could control the number and size of the filaments (e.g. I want to be able to put in five nucleators and get five filaments.). Also, we're making the monomers in cell-free E. coli extracts so it's likely that I will have trouble achieving the concentration necessary to make the filaments without nucleators. Speaking of E. coli, do you think that would be possible? I found that formins and also artificially constructed trimers can nucleate as well. And formins might be better than arp2/3 because it appears that arp2/3 is designed for dynamic lamellapodia isntead of something more static. I think that's why it makes the 70 degree angles. One more thing, do you know if phalloidin would cause undesired nucleation of my filaments? Thanks Amulekii 04:35, 19 May 2006 (UTC)

Something is wrong with the cookies on my computer, so I wasn't able to read that paper you refer to, but I'll look at it when I'm not on this computer. About controlling the length and number. Yeah. I know that's really hard. Actually I think anything even approaching a true monodispersity of length would be impossible without a template of pre-defined length, or perhaps nucleating them between two fixed caps that were a defined distance.

No x-ray structures yet? I've been looking for them. If you say they don't exist I'll search a bit more, then just believe you. What are the best models we have? I am interested in mutating the interface to try to enhance the binding (as I am working with low concentrations, and I'd rather not use phalloidin). I'd really like a model of the filament that is as good as possible. Also, Are the N and C termini exposed in F-actin? Sorry to ask you such basic questions, but I'm just starting this project, and there are still a lot of things I don't understand. I wanted to know if the termini are exposed because instead of Arp2/3 I wanted to nucleate with maybe a string of three monomers. You may already know that several years ago they were able to nucleate actin by cross-linking three monomers. I want to do something similar, which would be simply to link them (assuming the termini don't get in the way) which I imagine would siginficantly increase the likelihood of forming a trimer that could nucleate my filaments.

I can't tell if Mts are a better choice either. I'd like them because they're stronger, and I want to make stuff out of them. But my biggest challenge for now is that they both apparently need CCT to fold. Amulekii 22:27, 25 May 2006 (UTC)

Oh yeah. About my project. That's about it. I just want to make some kind of filament that can be controlled and stabilized and I want to do it using E. coli extracts. It's tough, but I guess that's good, or someone would have done it. Amulekii 22:29, 25 May 2006 (UTC)

I looked at both those papers. They look pretty interesting. I'll file them away as they may come in handy. How do you speak English so well? Amulekii 04:41, 26 May 2006 (UTC)

[edit] Why thank you!

Many thanks, i'm glad that someone else realizes this. Batman2005 03:08, 5 July 2006 (UTC)

You are welcome. I guess with the Championships the article got a lot of attention. But I have a feeling that some people are trying to push their agenda. The entire Trivia section is POV in my opinion. It only tries to state how polish Poloski is. But I don't want to remove it before discussing it on the discussion page. --Splette

^Talk 03:23, 5 July 2006 (UTC)

Well you've got my support in removing it, trivia sections are generally looked down on because if the information was truly encyclopedic, it would be elsewhere in the article in the first place. Batman2005 14:21, 5 July 2006 (UTC)

Yes, well at least they have a source for the allegation of the anthem now. Still, I think the article is POV. --Splette

^Talk 14:24, 5 July 2006 (UTC)

[edit] Hezbollah

There's no need to rediscuss the issue at the talk page. Just remove ot whenever you spot it. I haven't spotted it. Well done. Cheers -- Szvest 13:07, 22 August 2006 (UTC) ^{Wiki me up ®}

[edit] Template:Infobox Digicam image size

Hi, I suggest to discuss this topic in Template_talk:Infobox_Digicam#Suggestions--Marc Lacoste 08:35, 25 August 2006 (UTC)

[edit] Standardizing Protein Images

Errr, why was this entry created on your userpage and not at Molecular and Cellular Biology/Proposals?

I've no idea... have you been clicking things again? You idea is good.. if you havn't already, copy and paste it to proposals, to see if anyone argues a different program. Thanks for your interest. --Username132 (talk) 19:57, 31 August 2006 (UTC)

[edit] Re: Assessment of MCB articles

Hi, Splette. Much of the organization page is bot-constructed: I'll try to explain.

There is a bot, Mathbot, which uses the {{Wikiproject MCB}} templates on the various talk pages to build the Wikipedia:Version 1.0 Editorial Team/MCB articles by quality page. There is no need to manually add article to the Organization page.
An application I wrote takes all information on that page, and merges it with Wikipedia:WikiProject Molecular and Cellular Biology/Organization, as follows:
- The status, importance, and comments for all listings are copied directly from Mathbot's page. Any manual changes to the Organization page are lost.
- The "sections" on the Organization page are preserved, so you can freely move any number of listings from, for example "Fundamentals" to "Cell Biology".
- Any that exist in the Organization page but not in the Mathbot page are automatically labelled in its comments section as not having a {{Wikiproject MCB}} template.

Hope that helps, and if you have any more questions, feel free to ask! – Clockwork Soul 17:46, 16 October 2006 (UTC)

[edit] MCB WikiProject Page

The changes look good - thanks :) – Clockwork Soul 15:56, 19 October 2006 (UTC)

You are welcome, coordinator :) --Splette

^Talk 15:57, 19 October 2006 (UTC)

[edit] your signature

Hi, Splette! I really like that you've included a happy face in your signature. The project really could use a little more mirth, you know? But I also seem to remember reading earlier this year (when some of these signatures got really out of hand) that it was recommended not to have images in signatures. Do you know if that's been reversed? If not, it would be perfectly appropriate on your user page, of course. :) ... aa:talk 17:23, 20 October 2006 (UTC)

[edit] Muchas gracias

Hey Splette, thanks a lot for supporting me in my recent RfA. It succeeded, and I am very grateful to all of you. If you ever need help with anything, please don't hesitate to ask. Also, feel free point out any mistakes I make! Thanks again, —Khoi khoi 05:04, 21 October 2006 (UTC)

[edit] Molecular and Cellular Biology Wikiproject Newsletter

The project main page has gotten a facelift!
When people visit the project, the very first thing that they see tends to be the project's main page, and with this in mind, the main page has been completely overhauled. To enhance readability the various "goals" sections have been merged, and a detailed "how you can help" section has been added. To increase accessibility for more established members, the links to any resources that were in the main body text have been moved onto the navigation bar on the right. Finally, the whole page has been nicely laid out and given a nice attractive look.
New project feature: peer review
I'm proud to announce the addition of out newest feature: peer review! The MCB peer review feature aims serve as a stepping stone to improve articles to featured article status by allowing editors to request the opinions of other members about articles that they might not otherwise see or contribute to.
Project progress
The article worklist
We’ve had quite a bit of progress on the worklist article in the past month. Not only has the list itself nearly doubled in size from 143 to 365 entries, but an amazing three articles have been advanced to FA status, thanks in great part to the efforts of our very own TimVickers! Remember, the state of the worklist is the closest thing we have to quantifying the progress of the project, so if you get the chance, please take a look at the list, pick a favorite article, and improve it!
Collaboration of the Month
Last month's Collaboration of the Month, cell nucleus, was a terrific success! In one month, the article went from a dismal stub to an A-class article. Many thanks to all of the collaborators who contributed, especially ShaiM, who took on the greatest part of the burden. This month's Collaboration of the Month, adenosine triphosphate, isn't getting nearly the attention of its predecessor, so if you can, please lend a hand!
Finally...
The project has a new coordinator, Clockwork Soul! The role - my role - of coordinator will be to harmonize the project's common efforts, in part by organizing the various tasks required to make the project run as smoothly and completely as possible. Many thanks to those who supported me and those participated in the selection process.
Clockwork Soul, project coordinator 18:16, 21 October 2006 (UTC)
If you wish to opt out of having the newsletter posted on your talk page in the future, you may add yourself to the opt out list Newsletter concept and layout blatantly "borrowed" from the Esperanza newsletter.

[edit] Award

		The Original Barnstar
		For your hardwork and dedication to making Wikipedia a better place. I, Sharkface217, award you this Original Barnstar. Good job! :-)Sharkface217 04:37, 1 November 2006 (UTC)

[edit] This month's winner is proteasome!

Proteasome
This month's MCB Collaboration of the Month article is proteasome. Please help to improve this article to featured article status.
Last month's collaboration was adenosine triphosphate

– Clockwork Soul 22:23, 1 November 2006 (UTC)

[edit] Protein Representation Picture Requests

Since you volunteered, I took the liberty of adding this section to your discussion page, so we would all have a place to give you work to do. ;) So here are some protein representation picture requests for you to consider:

Bcl-2-associated_X_protein (BAX) [1] ~Doc~ EquationDoc 16:39, 10 November 2006 (UTC)
BH3_interacting_domain_death_agonist (BID) http://www.pdb.org/pdb/explore.do?structureId=2BID ~Doc~ EquationDoc 16:39, 10 November 2006 (UTC)

BTW, perhaps it would be better to add, say, Wikipedia:WikiProject Molecular and Cellular Biology/ProteinPictureRequests where all of those interested and capable (ie. not just you) could field such requests...I've added a comment along these lines to Wikipedia:WikiProject Molecular and Cellular Biology/Proposals. ~Doc~ EquationDoc 16:39, 10 November 2006 (UTC)

I agree, a more central place to file requests makes sense. Anyway, I uploaded an image for BAX. BID will follow... --Splette :) ^Talk 15:15, 11 November 2006 (UTC)

I just saw it--you rawk! ~Doc~ EquationDoc 15:24, 11 November 2006 (UTC)

By the way, I just created a protein request page, Wikipedia:WikiProject Molecular and Cellular Biology/Requested proteins so you can add it to your watchlist if you like, and we can stop cluttering up your talk page. :) I will add the BID entry there. ~Doc~ EquationDoc 16:25, 11 November 2006 (UTC)

[edit] Vielen Dank

Hey, thanks for your nice comment at Talk:Expulsion of Germans after World War II. Sca 16:34, 14 November 2006 (UTC)

[edit] Proteasome images

Hey - wondering if you'd mind creating another image for proteasome in the same style as the existing ones, but with alpha and beta subunits colored differently? (Possibly just with the 20S particle.) I think it would be useful to illustrate how the alpha-beta-beta-alpha stack is arranged. BTW, I also commented on your diagram suggestion on Talk:Proteasome, but it was a good week or so after your post. Opabinia regalis 04:47, 16 November 2006 (UTC)

[edit] This month's winner is RNA interference!

RNA interference
This month's MCB Collaboration of the Month article is RNA interference. Please help to improve this article to featured article status.
Last month's collaboration was proteasome

– Clockwork Soul 14:46, 2 December 2006 (UTC)

[edit] FAC for Bacteria

An editor has made some major changes to this article, could you please return to the FAC and provide some feedback on whether or not these are an improvement? TimVickers 21:22, 2 December 2006 (UTC)

[edit] Greetings

Merry Christmas, Joyeaux Noël, Frohe Weinachten, Wesołych Swiąt, Linksmai Kalėdos, Весёпый Рождествόм!

Sca 22:26, 24 December 2006 (UTC)

[edit] This month's MCB Collaboration of the Month article is Peripheral membrane protein!

Peripheral membrane protein
This month's MCB Collaboration of the Month article is Peripheral membrane protein. Please help to improve this article to featured article status.
Last month's collaboration was RNA interference

– Clockwork Soul 19:00, 14 January 2007 (UTC)

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