Talk:Shotgun sequencing
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- The DNA is first cut into small pieces by restriction enzymes.
because I feel certain that's wrong. If the DNA is cut, it's more likely fractionated, because it's important to get random samples. The article as a whole is pretty vague and somewhat suspect, but unfortunately I don't know that much about the details either...
Zashaw 23:11, 21 Jun 2004 (UTC)
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[edit] So how do you do the sequencing then?
There's something odd about this article: Sequencing lists this as a method used to sequence DNA, but this article says "...and then these clones are sequenced". How can the last step of sequencing be sequencing? Does it mean, "these clones are sequenced using sequencing method X"? Of the three methods mentioned under sequencing, only Chain termination method seems to actually be a method of sequencing, the other two just refer back to some unexplained "sequencing" process. Anyone care to clarify? - IMSoP 00:19, 22 Oct 2004 (UTC)
- Fixed (I hope I got it right). - IMSoP 21:21, 14 Nov 2004 (UTC)
[edit] Whole genome shotgun sequencing
What is the difference between this and Whole genome shotgun sequencing? Jmeppley 21:26, 26 May 2005 (UTC)
- The two articles should really be merged. An alternative to WGS is the BAC-based method...the difference is in how careful you are about splitting up the original DNA into pieces to be sequenced. But the basic idea of assembly is the same with either method, and the term "shotgun" is in contrast to chromosome walking. The simplified information presently in this article applies to both WGS and BAC approaches. (see for example [1]) --Mike Lin 08:54, 13 October 2005 (UTC)
[edit] Double Barrel Shotgun Sequencing
Some information about Double Barrel Shotgun Sequencing would be nice. T_P
reply (to Double Barrel Shotgun Sequencing": LOL man I'm a grad school student reading up about this stuff for a bioinformatics project and this really made my day!! haha -Kris
[edit] External Link to Scientist Article
Why is there a link to material that is not freely available? I don't think we should link to articles that require registration. neffk 17:36, 9 August 2006 (UTC)
[edit] Craig`s argument is that SPEED MATTERS
But that`s just really not true, as we know for a long time that even so much as one nucleotide pair can have a dramatic change, in fact a methylation alone can have an enourmous effect. So even the human genome project is barely enough in terms of quality. Whole genome shotgun sequencing is a top technology, no question about that but only to provide a first glimpse, similar to a page being scanned and getting a thumbail before the actual scanning progcess. In the future as software and our understanding of biology becomes better all this might change in favor of shotg. seq. but until then it remains as i pointed out.Slicky 12:26, 24 August 2006 (UTC)
[edit] Please provide reference
Please provide a reference for "For example, to complete the Human Genome Project, most of the human genome was sequenced at 12X or greater coverage; that is, each base in the final sequence was present, on average, in 12 reads. Even so, current methods have failed to isolate or assemble reliable sequence for approximately 1% of the (euchromatic) human genome.". Thank you. —The preceding unsigned comment was added by 130.212.235.23 (talk) 21:29, 20 March 2007 (UTC).
