Second Generation Multiplex Plus

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Second Generation Multiplex Plus - Manufactured by ABI (Applied Biosystems)

Updated version of and compatible with the Second Generation Multiplex DNA profiling system. DNA profiling system used by The UK National DNA Database from 1998 onwards.

Uses the same STR loci as in the SGM DNA profiling system, though uses different DNA primers, also contains DNA primers for an additional four STR loci (D3, D16, D2 and D19).

D3 (D3S1358), VWA (HUMVWF31/A), D16 (D16S539), D2 (D2S1338), D8 (D8S1179), D21 (D21S11), D18 (D18S51), D19 (D19S433), THO (HUMTHO1), FGA (HUMFIBRA)

Also contains primers for the Amelogenin (Amelo) sex indicating test.

The primers are tagged with the following fluorescent dyes for detection under electrophoresis.

  • 5-FAM
  • JOE
  • NED

The primers for each Loci are arranged on the dyes in the following order from low molecular weight to large molecular weight.

  • 5 - FAM. D3, VWA, D16, D2
  • JOE. Amelo, D8, D21, D18
  • NED. D19, THO, FGA

The dyes each primer is attached to differs to the SGM DNA profiling system.


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