Primer extension

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Primer extension is a technique whereby the 5' ends of RNA or DNA can be sequenced.

In this technique, we need an oligonucleotide from a transcribed DNA sequence. This oligonucleotide is hybrided on a mRNA. The oligonucleotide sequence is used as a primer for the reverse transcriptase. We can then know the length of the new synthetised cDNA from the oligonucleotide sequence to the transcription start point.

[edit] References

http://www.biochem.arizona.edu/classes/bioc568/primer_extension.htm

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