Polony
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Polony is a contraction of "polymerase colony," a small colony of DNA.
"Polonies are discrete clonal amplifications of a single DNA molecule, grown on a solid-phase surface. This approach greatly improves the signal-to-noise ratio. Polonies can be generated using several techniques that include solid-phase PCR in polyacrylamide gels, bridge PCR, rolling-circle amplification, BEAMing (beads, emulsions, amplification and magnetics)-based cloning on beads and massively parallel signature sequencing (MPSS) to generate clonal bead arrays." [1]
In one conceptually simple way to create polonies, a solution containing DNA fragments is poured onto a microscope slide dilute enough so that individual molecules are separated. DNA polymerase is added, which copies each fragment repeatedly, creating millions of polonies, each of which contains only copies of the original fragment of DNA. These "polonies" are then used for various kinds of DNA research like DNA sequencing.
[edit] References
- Polony technology guide
- Fan Jb, Chee, MS, Gunderson, KL (2006) Highly parallel genomic assays. Nature Reviews of Genetics (8):632-44.
- Zhang et al. Sequencing genomes from single cells by polymerase cloning.
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