Phenylalanine hydroxylase

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predicted 3D structure of phenylalanine hydroxylase
phenylalanine hydroxylase
Identifiers
Symbol	PAH
HUGO	8582
Entrez	5053
OMIM	261600
RefSeq	NM_000277
UniProt	P00439
Other data
EC number	1.14.16.1
Locus	Chr. 12 q22-q24.2

Phenylalanine hydroxylase (EC 1.14.16.1) is an enzyme which catalyses the reaction causing the addition of an hydroxyl group to the end of the 6-carbon aromatic ring of phenylalanine, such that it becomes tyrosine:

Phenylalanine

Tyrosine

Phenylalanine hydroxylase is the rate-limiting enzyme of the metabolic pathway which degrades excess phenylalanine.

The other substrates in the reaction are molecular oxygen and tetrahydrobiopterin. Tetrahydrobiopterin is a member of the group of redox biochemicals known as pteridines. PAH is the gene that encodes for phenylalanine hydroxylase.

[edit] Clinical significance

Mutations in phenylalanine hydroxylase which result in lower activity are the cause of the disease phenylketonuria, or PKU.

[edit] Related enzymes

Phenylalanine hydroxylase is closely related to two other enzymes:

• tryptophan hydroxylase (EC number 1.14.16.4), which controls levels of serotonin in the brain and the gastrointestinal tract
• tyrosine hydroxylase (EC number 1.14.16.2), which controls levels of dopamine, epinephrine, and norepinephrine in the brain and the adrenal medulla.

The three enzymes are homologous, that is, are thought to have evolved from the same ancient hydroxylase.

[edit] Structure

Phenylalanine hydroxylase is a tetramer composed of two dimers, that is, composed of two complexes of two subunits each. Each subunit is in turn composed of three domains, a regulatory domain, a catalytic domain, and a tetramerization domain.

• The regulatory domain is composed of the approximately 115 amino acids nearest the amino terminal of the subunit.

• The catalytic domain is composed of the next approximately 300 amino acids, and is responsible for all of the catalytic activity of the enzyme.

• The tetramerization domain consists of the remaining amino acids and through the formation of a coiled-coil arrangement of amino acids, holds the tetrameric structure of the holoenzyme together with a leucine zipper.

Phenylalanine hydroxylase contains one bound iron atom per subunit which is necessary for catalytic activity.