Nitrogen-15

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Nitrogen-15 is a stable, non-radioactive isotope of nitrogen. It is often used in agricultural and medical research. Nitrogen-15 is frequently used in nuclear magnetic resonance spectroscopy (NMR), because unlike the more abundant nitrogen-14, it has a net nuclear spin, which is required for NMR. Proteins can be isotopical labelled by cultivating them in a medium containing only nitrogen-15. This allows determination of the three dimensional structure of a protein by NMR spectroscopy. In addition, nitrogen-15 is used to label proteins in quantitative proteomics (e.g. SILAC).

It is a product of nitrogen-15 beta decay in stars.


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