Nitrate reductase test

From Wikipedia, the free encyclopedia

You have new messages (last change).
There are very few or no other articles that link to this one.
Please help introduce links in articles on related topics. After links have been created, remove this message.
This article has been tagged since November 2006.

The nitrate reductase test is a test to differentiate between bacteria based on their ability or inability to reduce nitrate (NO3) to nitrite (NO2) using anaerobic respiration.

[edit] Procedure:

  1. Inoculate nitrate broth with an isolate and incubate for 48 hours.
  2. Add 10-15 drops each of sulfanilic acid and N,N-dimethyl-1-naphthylamine. If the bacterium produces nitrate reductase, the broth will turn a deep red within 5 minutes at this step.
  3. If no color change is observed, then the result is inconclusive. Add a small amount of zinc to the broth. If the solution remains colorless, then both nitrate and nitrite reductase are present. If the solution turns red, nitrate reductase is not present.


 This biology article is a stub. You can help Wikipedia by expanding it.
Retrieved from "http://en.wikipedia.org../../../n/i/t/Nitrate_reductase_test.html"