Jubalio
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"Jubalio" is an upbeat folk tune popular during the American Civil War. A fairly obscure piece, it appeared in two cartoons by Tex Avery, "Billy-Boy" and "The Three Little Pups". The piece was whistled throughout both pictures by a dimwitted wolf voiced by Daws Butler (who used the same slow Southern drawl later for Huckleberry Hound).
John Wayne whistles this tune in the 1933 movie The Telegraph Trail.