Hot 100 number-one hits of 2001 (USA)
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This is a list of the U.S. Billboard magazine Hot 100 number-ones of 2001.
| Issue Date | Song | Artist |
| January 6 | Independent Women Part I | Destiny's Child |
| January 13 | Independent Women Part I | Destiny's Child |
| January 20 | Independent Women Part I | Destiny's Child |
| January 27 | Independent Women Part I | Destiny's Child |
| February 3 | It Wasn't Me | Shaggy featuring Ricardo "RikRok" Ducent |
| February 10 | It Wasn't Me | Shaggy featuring Ricardo "RikRok" Ducent |
| February 17 | Ms. Jackson | OutKast |
| February 24 | Stutter | Joe featuring Mystikal |
| March 3 | Stutter | Joe featuring Mystikal |
| March 10 | Stutter | Joe featuring Mystikal |
| March 17 | Stutter | Joe featuring Mystikal |
| March 24 | Butterfly | Crazy Town |
| March 31 | Angel | Shaggy featuring Rayvon |
| April 7 | Butterfly | Crazy Town |
| April 14 | All for You | Janet |
| April 21 | All for You | Janet |
| April 28 | All for You | Janet |
| May 5 | All for You | Janet |
| May 12 | All for You | Janet |
| May 19 | All for You | Janet |
| May 26 | All for You | Janet |
| June 2 | Lady Marmalade | Christina Aguilera, Lil' Kim, Mya and Pink |
| June 9 | Lady Marmalade | Christina Aguilera, Lil' Kim, Mya and Pink |
| June 16 | Lady Marmalade | Christina Aguilera, Lil' Kim, Mya and Pink |
| June 23 | Lady Marmalade | Christina Aguilera, Lil' Kim, Mya and Pink |
| June 30 | Lady Marmalade | Christina Aguilera, Lil' Kim, Mya and Pink |
| July 7 | U Remind Me | Usher |
| July 14 | U Remind Me | Usher |
| July 21 | U Remind Me | Usher |
| July 28 | U Remind Me | Usher |
| August 4 | Bootylicious | Destiny's Child |
| August 11 | Bootylicious | Destiny's Child |
| August 18 | Fallin' | Alicia Keys |
| August 25 | Fallin' | Alicia Keys |
| September 1 | Fallin' | Alicia Keys |
| September 8 | I'm Real (Murder Remix) | Jennifer Lopez featuring Ja Rule |
| September 15 | I'm Real (Murder Remix) | Jennifer Lopez featuring Ja Rule |
| September 22 | I'm Real (Murder Remix) | Jennifer Lopez featuring Ja Rule |
| September 29 | Fallin' | Alicia Keys |
| October 6 | Fallin' | Alicia Keys |
| October 13 | Fallin' | Alicia Keys |
| October 20 | I'm Real (Murder Remix) | Jennifer Lopez featuring Ja Rule |
| October 27 | I'm Real (Murder Remix) | Jennifer Lopez featuring Ja Rule |
| November 3 | Family Affair | Mary J. Blige |
| November 10 | Family Affair | Mary J. Blige |
| November 17 | Family Affair | Mary J. Blige |
| November 24 | Family Affair | Mary J. Blige |
| December 1 | Family Affair | Mary J. Blige |
| December 8 | Family Affair | Mary J. Blige |
| December 15 | U Got It Bad | Usher |
| December 22 | How You Remind Me | Nickelback |
| December 29 | How You Remind Me | Nickelback |
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