Hot 100 number-one hits of 1965 (USA)
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These are the Billboard magazine Hot 100 number one hits of 1965.
| Issue Date | Song | Artist |
| January 2 | I Feel Fine | The Beatles |
| January 9 | I Feel Fine | The Beatles |
| January 16 | Come See About Me | The Supremes |
| January 23 | Downtown | Petula Clark |
| January 30 | Downtown | Petula Clark |
| February 6 | You've Lost That Lovin' Feelin' | The Righteous Brothers |
| February 13 | You've Lost That Lovin' Feelin' | The Righteous Brothers |
| February 20 | This Diamond Ring | Gary Lewis & the Playboys |
| February 27 | This Diamond Ring | Gary Lewis & The Playboys |
| March 6 | My Girl | The Temptations |
| March 13 | Eight Days a Week | The Beatles |
| March 20 | Eight Days a Week | The Beatles |
| March 27 | Stop! In the Name of Love | The Supremes |
| April 3 | Stop! In the Name of Love | The Supremes |
| April 10 | I'm Telling You Now | Freddie and the Dreamers |
| April 17 | I'm Telling You Now | Freddie and the Dreamers |
| April 24 | Game of Love | Wayne Fontana and the Mindbenders |
| May 1 | Mrs. Brown, You've Got a Lovely Daughter | Herman's Hermits |
| May 8 | Mrs. Brown, You've Got a Lovely Daughter | Herman's Hermits |
| May 15 | Mrs. Brown, You've Got a Lovely Daughter | Herman's Hermits |
| May 22 | Ticket to Ride | The Beatles |
| May 29 | Help Me Rhonda | The Beach Boys |
| June 5 | Help Me Rhonda | The Beach Boys |
| June 12 | Back in My Arms Again | The Supremes |
| June 19 | I Can't Help Myself (Sugar Pie Honey Bunch) | The Four Tops |
| June 26 | Mr. Tambourine Man | The Byrds |
| July 3 | I Can't Help Myself (Sugar Pie Honey Bunch) | The Four Tops |
| July 10 | (I Can't Get No) Satisfaction | The Rolling Stones |
| July 17 | (I Can't Get No) Satisfaction | The Rolling Stones |
| July 24 | (I Can't Get No) Satisfaction | The Rolling Stones |
| July 31 | (I Can't Get No) Satisfaction | The Rolling Stones |
| August 7 | I'm Henry VIII, I Am | Herman's Hermits |
| August 14 | I Got You Babe | Sonny and Cher |
| August 21 | I Got You Babe | Sonny and Cher |
| August 28 | I Got You Babe | Sonny and Cher |
| September 4 | Help! | The Beatles |
| September 11 | Help! | The Beatles |
| September 18 | Help! | The Beatles |
| September 25 | Eve of Destruction | Barry McGuire |
| October 2 | Hang on Sloopy | The McCoys |
| October 9 | Yesterday | The Beatles |
| October 16 | Yesterday | The Beatles |
| October 23 | Yesterday | The Beatles |
| October 30 | Yesterday | The Beatles |
| November 6 | Get Off of My Cloud | The Rolling Stones |
| November 13 | Get Off of My Cloud | The Rolling Stones |
| November 20 | I Hear a Symphony | The Supremes |
| November 27 | I Hear a Symphony | The Supremes |
| December 4 | Turn! Turn! Turn! (To Everything There Is a Season) | The Byrds |
| December 11 | Turn! Turn! Turn! (To Everything There Is a Season) | The Byrds |
| December 18 | Turn! Turn! Turn! (To Everything There Is a Season) | The Byrds |
| December 25 | Over and Over | The Dave Clark Five |
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1940 | 1941 | 1942 | 1943 | 1944 | 1945 | 1946 | 1947 | 1948 | 1949 |
