Horseradish peroxidase
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The enzyme horseradish peroxidase, found in horseradish, is used extensively in molecular biology in antibody amplification and detection, among other things[1]. For example, "In recent years the technique of marking neurons with the enzyme horseradish peroxidase (HRP) has become a major tool. In its brief history, this method has probably been used by more neurobiologists than have used the Golgi stain since its discovery in 1870."[2] Horseradish peroxidase is also highly used in techniques such as Western blotting and ELISAs.
[edit] References
- ^ Chau YP, Lu KS (1995). "Investigation of the blood-ganglion barrier properties in rat sympathetic ganglia by using lanthanum ion and horseradish peroxidase as tracers". ACTA ANATOMICA (BASEL) 153 (2): 135-144. PMID 8560966.
- ^ Quoted in "Cell Marking with Horseradish Peroxidase" adapted from D. Purves and J.W. Lichtman, Principles of Neural Development. Sinauer Associates, Inc., Sunderland, 1985.
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Catalase - Cytochrome c peroxidase - Eosinophil peroxidase - Glutathione peroxidase - Horseradish peroxidase - Lactoperoxidase - Myeloperoxidase - Thyroid peroxidase - Deiodinase (Tetraiodothyronine 5' deiodinase)
