Fiber diffraction
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Fiber diffraction is a scattering technique in which molecular structure is determined from scattering data (usually of X-rays or electrons) from filaments composed of a regular array of molecules distinguished by a single direction (the fiber axis). The resulting diffraction patterns show layer lines, each with Bessel function intensities.
[edit] Historical role
Fiber diffraction data led to several important advances in the development of structural biology, e.g., the original models of the α-helix and the Watson-Crick model of double-stranded DNA.
[edit] References
Cochran W, Crick FHC and Vand V. (1952) "The Structure of Synthetic Polypeptides. I. The Transform of Atoms on a Helix", Acta Cryst., 5, 581-586.
| Protein structure determination methods | ||
|---|---|---|
| High resolution: | X-ray crystallography | NMR | Electron crystallography | |
| Medium resolution: | Cryo-electron microscopy | Fiber diffraction | Mass spectrometry | |
| Spectroscopic: | NMR | Circular dichroism | Absorbance | Fluorescence | Fluorescence anisotropy | |
| Translational Diffusion: | Analytical ultracentrifugation | Size exclusion chromatography | Light scattering | NMR | |
| Rotational Diffusion: | Fluorescence anisotropy | Flow birefringence | Dielectric relaxation | NMR | |
| Chemical: | Hydrogen-deuterium exchange | Site-directed mutagenesis | Chemical modification | |
| Thermodynamic: | Equilibrium unfolding | |
| Computational: | Protein structure prediction | Molecular docking | |
| ←Tertiary structure | Quaternary structure→ | |
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