User:F. Cosoleto/Report

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[edit] Czerkinsky, Cecil

  • google - http://www.pac.ne.jp/aifii/cecil.html
    • Before joining the International Vaccine Institute, Dr. Czerkinsky has been serving as the Director of the Division of Mucosal Immunology and Vaccinology at INSERM (French National Institute of Health and Medical Research) since 1997, focusing on basic mechanisms governing induction of immunity in mucosal organs.
  • google - http://www.pac.ne.jp/aifii/cecil.html
    • Dr. Czerkinsky received doctoral degrees from the University of Lyon Medical and Dental Faculty in France, and the University of Gothenburg Medical School in Sweden. He has more than 130 scientific peer-reviewed publications in immunology and vaccinology with a major focus on mucosal vaccines and immunomodulators.
  • google - http://www.pac.ne.jp/aifii/cecil.html
    • Dr. Czerkinsky invented the ELISPOT, a method for monitoring immune responses in humans and animals, which is used world-wide. His work has been focused on basic immunological mechanisms pertaining to vaccine development against infections, auto-immune disorders and allergies.

[edit] ELISPOT

  • google, yahoo - http://www.immunospot.com/science-elispot-assays.html
    • Moreover, ELISPOT assays measure actually secreted molecules in pharmacologically untreated cells. This accounts for post-transcription regulation, thereby visualizing the biologically relevant products.
  • google - http://www.immunospot.com/science-elispot-assays.html
    • Cytokine production can also be detected by intracytoplasmic staining and flow cytometry. Like ELISPOT, intracytoplasmic staining provides information on the actual frequency of antigen-specific T cells that produce a given cytokine. However, ELISPOT assays are orders of magnitude more sensitive in detecting low-frequency antigen-specific cells. Antigen-specific T cells frequently occur in frequencies lower than 1 in 10,000 in peripheral blood, placing them below the detection limit of flow cytometry but easily detectable via ELISPOT.
  • google - http://www.immunospot.com/science-elispot-assays.html
    • Unlike ELISPOT assays, cytokine bead arrays and ELISA assays do not provide frequency information and do not quantify the per-cell secretory activity. In contrast, ELISPOT assays can distinguish between few cells secreting large quantities of a molecule versus numerous cells secreting smaller quantities.
  • google - http://www.immunospot.com/science-elispot-assays.html
    • Unlike mRNA measurements that reflect cytokine expression at a single moment, ELISPOT assays capture the secretory product during the entire duration of the assay. This makes detection via ELISPOT insensitive to the fine kinetics of the cellular response. For the same reason, two-color ELISPOT assays are ideally suited for measuring the coexpression of different molecules by the same cell.
  • google - http://www.immunospot.com/science-elispot-assays.html
    • Cytokine and granzyme B ELISPOT assays have recently emerged as the most sensitive and robust technique for direct ex vivo monitoring of antigen-specific CD4 or CD8 T cells. By measuring the frequencies and cytokine signatures of individual cells, they establish the clonal size and effector class of the specific T cell pool.

[edit] Protein A

[edit] Gonorrhoea

[edit] Ectogenesis

[edit] Immunodiagnostics

  • google - http://www.rapid-diagnostics.org/tech-lateral.htm
  • google - http://www.healthcarehometest.com/
    • ICS tests are used for the specific qualitative or semi-quantitative detection of many analytes including antigens, antibodies, and even the products of nucleic acid amplification tests. One or several analytes can be tested for simultaneously on the same strip. Urine, saliva, serum, plasma, or whole blood can be used as specimens. Extracts of patient exudates or fluids have also been successfully used.
  • google - http://www.rapid-diagnostics.org/tech-lateral.htm
  • google - http://www.healthcarehometest.com/
    • Test specificity can also be very high. The tests use colloidal gold, dye, or latex bead conjugates to generate signal. The assembled strips are dried and packaged, making them stable for months or years when properly protected from moisture and excessive heat.
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