Crude lysate
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A crude lysate is the solution produced when cells are destroyed by disrupting their cell membranes in a process known as cytolysis. Crude lysates are routinely produced in biochemistry and cell biology laboratories during the process of protein purification, although purified cellular organelles can also be retrieved from the solution. The first step in processing a crude lysate is often ultracentrifugation, which separates the solution into distinct bands containing organelles, membrane lipids, proteins, and nucleic acids.
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