Talk:Circular dichroism
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Can anyone tell me if CD spectroscopy has been tested on polymers, and not only on biological materials ? thank you. Guillom 13:59, 4 October 2005 (UTC)
I think the link to ellipticity that is redirected to the flattening article should be removed because this parameter is different than the definition of elliptical polarization. --Bjsamelsonjones 6/28/06
Q: In the 'Experimental limitations' is this 0.1 mm pathlength correct? Or should it be 1 mm? Thanks A: It is correct. Both 1mm and 0.1 mm cuvettes are used. 0.1 mm cuvettes reduce the light scattering, leading to better spectra.
Cells are available with path lengths to .05mm (one cell manufacturer) , and shorter paths may sometimes be used; there are practical difficulties, e.g. knowing the true path length, sealing the mating faces so that evaporation does not change the concentration. Non-demountnable cells also exist. They can be very difficult to clean, and are quite expensive. Not only is high-purity syntheic quartz needed, but the cells should be strain-free, and the faces should be parallel. The benefit of short paths is more related to lower solvent absorbtion, as mentioned in the article, than to scattering. It is a challenge to find an appropriate solvent for work below 200nm. Curtis Johnson would be one name to search for in the literarture to learn about appropriate techniques.
--AJim 04:40, 25 March 2007 (UTC)
