CFSE

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CFSE (Carboxyfluorescein diacetate, succinimidyl ester) is a cell permeable dye that enters cells, covalently binds to intracellular amines and yields fluorescence after cleaved by intracellular esterases. Once the cell divides the dye remains in the two daughter cells at a lower concentration. A cell stained with CFSE can be kept in culture for several days. Each fluorescent peak represents another round of cell division. The area of each peak is the number of cells in that particular cycle. [1]

[edit] Reference

  1. ^ http://www.beckmancoulter.com/products/instrument/cellanalysis/celllab_quanta_sc_dcr.asp
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