Talk:Cell culture

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    I'm giving this article some attention, but welcome anyone else who would like to work on expanding it. Courtland 02:25, 2005 Mar 13 (UTC)

    Contents

    [edit] Article organization

    current thought on organizing the article:

    • Introduction (present)
    • Key concepts (begun)
    • Uses of cell culture
    • Specific examples
    • Notes & references (begun)
    • External links to methods and manuals
    • Related topics

    I'm thinking that all types of cell culture could be addressed in a general sense, with specific articles spinning off for things like insect cell culture, plant tissue culture, culture of anaerobic bacterial pathogens, viral culture, etc. Courtland 02:29, 2005 Mar 13 (UTC)


    I don't think that there needs to be much mention of microbial culturing. Make a link to microbiology, fermentation etc. In my experience cell culture in it's modern sense means, to the laboratory scientist, mainly animal and plant cell culture. Microbial cell culture is a term one hardly ever hears.--Alun 06:04, 11 May 2005 (UTC)

    [edit] Related articles (etc.) to consider while writing

    (not articles yet)

    [edit] image requests

    some might/should go into related articles Courtland 02:50, 2005 Mar 13 (UTC)

    		 It is requested that an image be included in this article to improve its quality, if possible. This image request is for large scale bacteria or yeast liquid culture bacterial petri culture, mammalian plate culture, basic materials required to prepare agar plates, basic tools required for bacterial culture, basic tools required for mammalian culture.

    [edit] Morpholino

    Sorry for my ignorance, but what is the relevance of this section to "cell culture"? Kaisershatner 18:32, 18 April 2006 (UTC)

    "Preclinical research with Morpholino antisense oligos has shown efficacy of the antisense against influenza in cell cultures. Cultures of African green monkey kidney cells (vero cells) were pretreated with Morpholino antisense oligos conjugated with arginine-rich peptides to enhance penetration of the oligos into the cytosol. Targeting translation-blocking Morpholinos against the nucleoprotein 1 mRNA or one of the polymerase protein mRNAs caused 2-3 log10 reductions in influenzavirus titer three days post-infection. When Morpholinos were administered post-infection, less antiviral activity was measured. [1]
    AVI BioPharma reports that when tested against several influenza strains by several independent laboratories, Morpholino oligos have suppressed viral replication and in one cse both replication and transcription were repressed. Co-administration of several Morpholino sequences caused as much as eightfold improvement in antiviral activity. [2]
    Can you explain why it doesn't belong in cell culture? Don't the two paragraphs talk about cell culture? If you are saying other stuff is more directly relevant, then my response is, well add that more directly relevent stuff and then the less directly relevant stuff can be removed at that time. If you are saying, no it really isn't even indirectly related, then you must know more about this than I do (which wouldn't be hard, I know very little about it); in which case we just keep it removed. WAS 4.250 19:31, 18 April 2006 (UTC)

    [edit] op.cit

    Shouldn't "op.cit" contain more information? WAS 4.250 19:23, 18 April 2006 (UTC)

    [edit] Cell culture and Tissue culture fusion

    For something that has so many applications in agriculture, medicine, and basic research both articles are extremely weak. The tissue culture should have the history and animal-plant distinctions and organ,tissue, cell distinction. I can see the cell culture needs to be its own article but is it at that stage presently or should it be fused with the weaker Tissue culture? The history of tissue culture could be a distinct article with the history of methodologies in culture, synchronizing cells, labeling cells, heterokaryons, hybridomas, suspension, substrates, transformation, contact inhibition, Hayflick senescence and now telomeres, SV-40 virus, etc. GetAgrippa 19:05, 26 January 2007 (UTC)

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